| Arsenic Trioxide Inhibits Proliferation in K562 Cells by Changing Cell Cycle and Survivin Expression |
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| 作者姓名： | 伍晓菲 陈智超 刘仲萍 周浩 游泳 黎纬明 邹萍 |
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| 作者单位： | DepartmentofHematology,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China |
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| 摘 要： | To study the mechanisms involved in the inhibition of chronic myeloid leukemic cells (K562) proliferation induced by arsenic trioxide (As2O3) and to explore the potential role of Survivin, an inhibitor of apoptosis protein, in the regulation of As203 induced cell apoptosis, K562 cells were cultured with As203 of different concentrations. Cells were collected for proliferation analysis by MTT assay. Cell cycle distribution and cell apoptosis were analyzed by flow cytometry. Expression of Survivin protein and mRNA were detected by flow cytometry and RT-PCR, respectively. Our results showed that As2O3 (2-10/μmol/L) inhibited K562 cells growth effectively, but it did not induce cells apoptosis significantly. The percentage of K562 cells at G2/M phase increased in proportion to As2O3 concentrations, and the expression of Survivin mRNA and content of Survivin protein was up-regulated accordingly. It is concluded that As2O3 inhibited K562 cells growth by inducing cell cycle arrest mainly at G2/M phase. Over-expression of Survivin gene and protein might be one of the possible mechanisms contributing to K562 cells‘ resistance to As2O3-induced apoptosis.
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| 关 键 词： | 三氧化二砷 抑制作用 分芽增殖 K562细胞 细胞转化 基因表达 As2O3 抑制剂 G2／M细胞周期停滞 Survivin 前髓细胞白血病 |
| 收稿时间： | 30 November 2003 |
Arsenic trioxide inhibits proliferation in K562 cells by changing cell cycle and survivin expression |
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| Wu?Xiaofei,Chen?Zhichao,Liu?Zhongping,Zhou?Hao,You?Yong,Li?Weiming,Zou?Ping.Arsenic Trioxide Inhibits Proliferation in K562 Cells by Changing Cell Cycle and Survivin Expression[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2004,24(4):342-344. |
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| Authors: | Wu Xiaofei Chen Zhichao Liu Zhongping Zhou Hao You Yong Li Weiming Zou Ping |
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| Institution: | Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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| Abstract: | Summary To study the mechanisms involved in the inhibition of chronic myeloid leukemic cells (K562) proliferation induced by arsenic
trioxide (As2O3) and to explore the potential role of Survivin, an inhibitor of apoptosis protein, in the regulation of As2O3 induced cell apoptosis, K562 cells were cultured with As2O3 of different concentrations. Cells were collected for proliferation analysis by MTT assay. Cell cycle distribution and cell
apoptosis were analyzed by flow cytometry. Expression of Survivin protein and mRNA were detected by flow cytometry and RT-PCR,
respectively. Our results showed that As2O3 (2–10 μmol/L) inhibited K562 cells growth effectively, but it did not induce cells apoptosis significantly. The percentage
of K562 cells at G2/M phase increased in proportion to As2O3 concentrations, and the expression of Survivin mRNA and content of Survivin protein was up-regulated accordingly. It is concluded
that As2O3 inhibited K562 cells growth by inducing cell cycle arrest mainly at G2/M phase. Over-expression of Survivin gene and protein might be one of the possible mechanisms contributing to K562 cells
resistance to As2O3-induced apoptosis.
WU Xiaofei, female, born in 1978, Graduate Student |
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| Keywords: | arsenic trioxide K562 G 2/M cell cycle arrest Survivin |
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