外周血CD4+CD25+调节性T细胞与乙型肝炎疫苗接种后应答关系的研究

目的 通过检测乙型肝炎(简称乙肝)疫苗接种后弱应答和无应答人外周血CD4+CD25+ 调节性T细胞(CD4+ CD25+ regulatory T cell,简称Treg细胞)的频率,探讨CD4+ CD25+ 调节性T细胞与乙肝疫苗接种后弱和(或)无应答之间的关系.方法 利用流式细胞分析方法 对25例乙肝疫苗无应答、30例乙肝疫苗弱应答者外周血Treg细胞的频率进行分析,另外选取20例乙肝疫苗强应答者作为对照.结果 强应答组Treg细胞的频率为(4.32±1.21)%,弱应答组Treg细胞的频率为(7.01±1.06)%,无应答组Treg细胞的频率为(12.75±2.01)%,无应答组和弱应答组与强应答组相比Treg细胞频率显著升高(t=8.426,t=3.289,P值均＜0.01).结论 乙肝疫苗接种后无应答和弱应答现象与外周血CD4+ CD25+ 调节性T细胞频率升高存在一定关系.

The relationship between CD4+ CD25+ regulatory T cell in the peripheral blood and poor or non-responsiveness to Hepatitis B vaccine

Objective To investigate CD4+ CD25+ regulatory T cell frequencies in the peripheral blood of poor or non-responsiveness to Hepatitis B vaccine,and try to understand the relationship between CD4+ CD25+ regulatory T cell and poor or non-responsiveness to Hepatitis B vaccine.Methods Flow cytometric analysis was employed for CD4+ CD25+ regulatory T cell frequencies in the peripheral blood of 25 cases of non-responsiveness.30 cases of poor-responsiveness.and coilected 20 cases of responsiveness as control.Results CD4+ CD25+ regulatory T cell frequencies of responsiveness was (4.32±1.21)%,poorresponsiveness was(7.01±1.06)% and non-responsiveness was (12.75±2.01)%.It was found that non and poor-responsiveness showed a high percentage of CD4+ CD25+ regulatory T cell as compared with responsiveness (t=8.426,t=3.289,P＜0.01).Conclusion The poor and non-responsiveness should be related with the increase of CD4+ CD25+ regulatory T cell and this might be considered as an important cause of poor and non-responsiveness.