中频磁场对小鼠血液及重要脏器的影响

目的探讨中频磁场对小鼠血液指标、重要脏器及生殖系统功能的影响。方法20只雄性昆明小鼠，体重（30±2）g，随机分为辐照组和对照组，每组10只。辐照组小鼠受磁场（40kHz，28mT，2h／d）连续照射7d。第8天检测血常规，取脑、肝、肾、脾及睾丸称重后进行组织分析。另24只雄性昆明小鼠分成4组，每组6只，包括对照组和实验1组（35mT，2h／d）、实验2组（35mT，1b／d）、实验3组（0．18mT，2h／d），实验组均连续照射7d，然后进行精子活率、精子密度检测。结果辐照组与对照组小鼠血常规及脏器称重结果差异均无显著性（P〉0．05）。病理切片提示脑、肝、肾和脾无病理变化，而睾丸小叶中各级生精细胞均有减少，精子细胞减少且排列紊乱。精子活率对照组为（40．58±7．75）％，实验1组降低至（7．40±1．06）％（q=16．909，P〈0．05），实验2组降低至（12．26±0．87）％（q=14．432，P〈0．05），实验3组（37．84±5．52）％与对照组差异无显著性（q=1．396，P〉0．05）。各实验组与对照组间精子密度差异无显著性（P〉0．05）。结论中频交变磁场对小鼠雄性生殖系统可能存在一定影响。睾丸可能是电磁辐射靶器官之一，对中频磁场（40kHz）极其敏感。而该条件下电磁场对小鼠血液及脑、肝、肾、脾等器官无明显影响。

Effects of Intermediate Frequency Alternating Magnetic Field on Blood Analysis and Histopathological Analysis for Important Organs in Mice

Objective To observe the effect of intermediate frequency （IF） alternating magnetic field on blood analysis, histopathological analysis for important organs and genital system in mice. Methods Twenty male Kunming mice weighed （30 ± 2） g were randomly divided into radiation group and control group （10 mice in each）. In the radiation group, the mice were treated in a magnetic field （28 mT,40 kHz, 2 hour per day） for 7 days. At day 8, blood routine test was performed, and the brain, liver, kidney, spleen, and testicle tissues were obtained for histopathological analysis. Meanwhile, another 24 male Kunming mice were divided randomly into 4 groups （6 in each） ： control group, experiment group 1 （35 mT, 2 h/d）, experiment group 2 （35 mT, 1 h/d）, and experiment group 3 （0.18 mT, 2 h/d）. All of the 3 experiment groups received radiation for 7 days. Afterwards, the sperm motility and sperm density of the mice were detected. Results No significant differences were found in the blood routine and weight of the organs among the experiment and control groups （ P 〉 0. 05 ）. Histopathologieal examination showed no pathological change in the brain, liver, kidneys, and spleen of the mice, but reduced spermatogenic cells spermatozoa. The sperm motility was （40.58 ± 7.75）% in the control, （7.40 ±1.06）% in the experiment group 1 （q =16.909, P 〈0.05）, （12.26 ±0.87）% in the the experiment group2 （q= 14. 432,P 〈0.05）, and （37.84 ±5.52）% in the experiment group 3 （q = 1.396, P〉0.05）. No significant difference in the sperm densities was found among the groups. Conclusions Intermediated alternating magnetic field has adverse effects on the reproduction of mice. Testosterone is one of the target organs of electromagnetic radiation. It is sensitive to IF magnetic field （40 kHz, 28 mT）. Blood, brain, liver, kidney and spleen of mice are not effected by IF alternating magnetic field.