mRNA差异显示法研究60Coγ辐射损伤内皮细胞相关基因

目的 筛选与鉴定电离辐射损伤相关的内皮细胞基因。方法 利用mRNA差异显示技术，以正常人脐静脉内皮细胞（HUVEC）和电离辐射后的HUVEC为实验材料，筛选与^60Coγ电离辐射损伤相关的基因片段。结果 经测序和鉴定，发现基因KIAA0456的两个外显子STSs和GSSs，ARF3基因在辐射后的内皮细胞中表达增加。结论 内皮细胞由上述两基因介导的细胞分化，信号传导，细胞粘附及增殖功能在辐射后增强。

Recognization and identification of genes correlative to ionization radiation in endothelial cells

Objective To screen and identify the genes in endothelial cell(EC) injury induced by ionizing radiation. Methods Using the technique of mRNA differential display,we screened the genes correlative to ionizing radiation in EC. Results Two exons-STSs and GSSs in KIAA0456 gene and ARF3 gene were endancively expressed in irradiated endothelial cells. Conclusion The function of cell division,signal transduction,cell adhesion and proliferation induced by the above two genes are all increased in irradiated endothelial cells. ;