| 中国人G6PD基因EcoRI酶切位点研究 |
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| 引用本文： | 许卫明,杜传书.中国人G6PD基因EcoRI酶切位点研究[J].中山大学学报(医学科学版),1993(2). |
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| 作者姓名： | 许卫明 杜传书 |
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| 作者单位： | 中山医科大学医学遗传学教研室
(许卫明)，中山医科大学医学遗传学教研室(杜传书) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 应用DNA Southern印迹杂交技术,以G6PD cDNA为探针,研究了16种限制酶的限制性片段长度多态,结果表明除EcoRⅠ外,其余15种限制酶均无多态改变,分析39例正常人与38例G6PD缺乏者G6PD基因ECO RⅠ酶切位点时,发现约20％的人出现7·0 kb的片段,1例G6PD患者出现6.0 kb片段,国外并无类似报道,并对这些片段来源及意义进行初步探讨。
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| 关 键 词： | 葡萄糖6磷酸脱氢酶基因 限制酶 EcoRⅠ DNA琼脂糖电泳 印迹杂交 限制性片段 长度多态 |
THE STUDY OF EcoR I SITE IN G6PD GENE FROM CHINESE |
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| Xu Weiming Du Chuanshu.THE STUDY OF EcoR I SITE IN G6PD GENE FROM CHINESE[J].Journal of Sun Yatsen University(Medical Sciences),1993(2). |
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| Authors: | Xu Weiming Du Chuanshu |
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| Institution: | Department of Medical Genetics |
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| Abstract: | The restriction fragment length polymorphisms (RFLP) of sixteen restriction endonuc-leases in G6PD gene were identified by using Southern blotting. Fifteen endonucleases were found no changes except for EcoR I . The results of 39 cases of normal individuals and 38 cases of G6PD deficients for G6PD gene digested by EcoR I showed that 20% individuals revealed 7.0 kb band, one G6PD deficient was 6.0 kb band, which were different from the abroad reports. The origin and significance for these fragments were discussed in this paper. |
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| Keywords: | G6PD gene restriction endonuclease EcoR I DNA agarose electrophoresis Southern blotting restriction fragment length polymorphism |
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