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| 小鼠肝细胞的分离与原代培养 | |
| 引用本文： | 赖静,杨天燕,韦锦斌,王乃平.小鼠肝细胞的分离与原代培养[J].广西医科大学学报,2011,28(5):806-807. |
| 作者姓名： | 赖静 杨天燕 韦锦斌 王乃平 |
| 作者单位： | 1. 广西中医学院药学院 南宁 530001 2. 广西医科大学第一附属医院药剂科 3. 广西医科大学药学院 |
| 摘 要： | 目的：探求一种简易、经济的小鼠原代肝细胞的分离与培养方法。方法：采用非灌注法分离小鼠肝脏,利用0.2%Ⅳ型胶原酶对肝脏消化来获取肝细胞,以DMEM培养基对肝细胞进行单层培养。结果：比较成功地进行了原代肝细胞培养,并进行了形态学观察。结论：此方法适合一般实验室开展小鼠原代肝细胞的分离与培养,为进一步的实验研究提供了基础。 |
| 关 键 词： | 原代细胞培养 肝细胞 细胞分离 细胞培养 |
ISOLATION AND PRIMARY CULTURE OF MURINE HEPATOCYTES |
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| Lai Jing,Yang Tianyan,Wei Jinbin,Wang Naiping.ISOLATION AND PRIMARY CULTURE OF MURINE HEPATOCYTES[J].Journal of Guangxi Medical University,2011,28(5):806-807. | |
| Authors: | Lai Jing Yang Tianyan Wei Jinbin Wang Naiping |
| Institution: | .(Guangxi Traditional Medical Universtity,Nanning 530001,China) |
| Abstract: | Objective：To search for an easier and cheaper method for the primary culture of murine hepatocytes.Methods：Isolating the murine liver by non-perfusion,using 0.2% IV Collagenase to digest the liver to get the primary hepatocytes,and culturing them with DMEM medium in the monolayer.Results：By this way,the primary hepatocytes culture was fomed successfully,and the hepatocytes were proved in shape.Conclusion：The method is good for the general labs to culture the hepatocytes,and it could provide the basic for the further research. |
| Keywords: | primary cell culture hepatocytes cell isolation cell culture |
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