耐甲氧西林金黄色葡萄球菌spa基因分型

目的 研究浙江省瑞安地区耐甲氧西林金黄色葡萄球菌(MRSA)的spa基因分型。 方法 采集2011年3-11月瑞安市人民医院检验科微生物室临床分离的金黄色葡萄球菌菌株,共100株。采用头孢西丁纸片法筛选耐甲氧西林金黄色葡萄球菌,PCR扩增MRSA spa基因的X区,测序后通过数据库进行分型。 结果 48株确证为MRSA,可分为16个型别,其中t030型15株,t437型12株,t6944型5株,t172型3株,t9538型2株,t5699、t148型、t179型、t1751型、t015型、t5554型、t127型、t159型、t062型、t163型各1株,同时发现1个新型t10149。 结论 瑞安地区MRSA的spa分型以t030和t437为主,t10149为新型菌种。

Study on spa typing of methicillin resistant Staphylococcus aureus

Objective To understand the types of spa gene of methicillin resistant Staphylococcus aureus（MRSA） in Rui＇an,Zhejiang province.Methods MRSA strains were selected by K-B test from 100 Staphylococcus aureus strains isolated in our hospital from March to November 2011.The X region of the strains＇ spa gene was amplified with PCR and genotyping was conducted after sequencing.Results Totally 48 MRSA strains were identified,which belonged to 16 genotypes,type t030 was detected in 15 strains,t437 in 12 strains,t6944 in 5 strains,t172 in 3 strains,t9538 in 2 strains and t5699,t148,t179,t1751,t015,t5554,t127,t159,t062 and t163 in 1 stain respectively.Meanwhile a new spa type（t10149） was detected.Conclusion The major spa types of MRSA are t030 and t437 in Rui＇an and t10149 is a new type.