一种新的老年期痴呆大鼠模型的研究

目的:建立一种新的用于老年期痴呆发病机制与防治研究的老年期大鼠模型。方法:雄性Wistar大鼠,双侧颈总动脉永久性结扎(2VO)并腹腔注射D-半乳糖(D-gal)6周(60mg/kg/天),制备老年期痴呆大鼠模型。采用神经电生理(事件相关电位)检测,脑组织切片Bielschowsky改良法银染色、HE染色、海马乙酰胆碱酯酶(AchE)活性检测等方法比较模型鼠和正常对照鼠的差异。结果:与对照组相比,模型组鼠出现白内障改变及明显P300潜伏期延长、脑内AchE活性降低、海马神经元变性、固缩、坏死及神经纤维紊乱等变化。结论:双侧颈兔动脉永久性结扎加D-gal腹腔注射制备的大鼠模型模拟了人类老年期痴呆的发病特点,可用于血管性痴呆、阿尔茨海默病等老年期痴呆的形态、病理生理变化机制及防治措施的基础与临床研究。

Research on a Rat Model of Aged Dementia

Objective:To establish a novel model of senile dementia in rats.Method:Wistar male rats were treated with a permanent bilateral occlusion of both carotid arteries(2-VO)and D-galactose(60 mg/kg/d,ip,42 d)to make senile dementia model in rats.The recording of event-related potential(ERP),the H&E staining and the modified Bielschowsky silver stain of paraffin sections,as well as the analysis of AchE activity in hippocampus were carried out to evaluate the rat model,compared with the normal rats.Results:As compared with the control group,the model groups showed the lagging of P300 in ERP,the decreased AchE activity in hippocampus,the degeneration of hippocampal and cerebral cortex neurons,and the neurofibrillary changes.Conclusion:Wistar rats treated with both 2-VO and D-galactose simulated some characteristic of human senile dementia,which may be used in basic experiment studying of senile dementia,such as vascular dementia,Alzheimer's disease and mixed dementia.