淋球菌耐药性质粒的转移性研究

目的研究淋病奈瑟菌(简称淋球菌)所携带的质粒与耐药性之间的关系。方法对淋球菌耐药株所携带的质粒进行质粒消除实验,并以此消除的质粒转化感受态的大肠杆菌,比较耐药质粒消除和转化前后菌株的耐药性变化情况。结果在亚致死浓度的十二烷基磺酸钠(SDS)作用下,淋球菌的耐药质粒可以被部分消除(仍然携带42.5kb的质粒)或者完全消除,且消除子在消除前后对抗生素的耐药性发生不同程度的改变;而耐药质粒在不同种属的菌株之间可进行传递,淋球菌的耐药质粒可以传递给感受态的大肠杆菌,筛选出的转化子分别携带含有tetM基因的42.5kb四环素耐药质粒和携带含有TEM-1基因的7.4kb青霉素耐药质粒,转化子可产生对相应抗生素的耐药性。结论淋球菌耐药质粒在介导菌株对抗生素的耐药性起着重要作用,并且可以不同菌株间进行传递。

The Research on the Transfer of the Drug Resistance Plasmid of N. gonorrhoeae

Objective To explore the relationship between the plasmids and resistance of N.gonorrhoeae.Methods plasmids hosted in one N.gonorrhoeae isolate were cured and the removed plasmids were transformed into competent E.coli strain DH5α.The antibiotic-resistant levels were compared before and after the removing of plasmids harbored in the N.gonorrhoeae isolate or the introducing of the plasmids into E.coli strain.Results The result showed that N.gonorrhoeae lost its plasmid either totally or partially under the SDS treatment in sub-lethal concentration.After removing the plasmid,the antibiotic-resistant level of the N.gonorrhoeae isolate was decreased.After introducing the plasmid into E.coli strain,the antibiotic-resistant level of E.coli strain was increased.Conclusion The data suggests that plasmid plays an important role in mediating drug resistance of N.gonorrhoeae and could be exchanged between different strains