肺癌细胞与血液细胞ERCC1基因的表达及其临床意义

目的 探讨铂类药物相关的切除修复交叉互补基因1（ERCC1） mRNA在不同病理组织学类型肺癌细胞及其对应患者血液细胞中的表达水平和临床意义。方法 收集105例肺癌标本石蜡组织及其对应的血液样本，其中腺癌73例，鳞状细胞癌22例，小细胞癌10例，利用实时荧光定量PCR技术检测肺癌石蜡组织和对应血液样本中ERCC1 mRNA的表达水平，分析其在肺癌不同病理组织学类型中的表达差异。结果105例肺癌石蜡组织中ERCC1 mRNA表达为4.579±1.926，对应的血样中为8.324±1.280，差异有统计学意义（P＜0.05）。ERCC1 mRNA在鳞状细胞癌、腺癌、小细胞癌中的表达分别为4.366±1.822、4.724±1.434和4.366±1.822，差异无统计学意义；在对应的血液细胞中表达分别为8.146±1.501、8.336±1.928和8.664±1.848，差异亦无统计学意义。3种病理组织学类型肺癌细胞ERCC1 mRNA表达水平与对应血液细胞相比，差异均有统计学意义（P＜0.05）。肺癌细胞和血液细胞中ERCC1 mRNA表达水平无相关性（r=0.329，P＞0.05）。结论 根据肺癌病理组织学类型选择铂类化疗并无指导意义，需要进一步进行ERCC1 mRNA的个体化检测，目前尚不能用血液细胞代替肿瘤组织细胞检测ERCC1 mRNA表达水平以评价铂类药物的疗效及毒副反应。

ERCC1 gene expression in lung cancer tumor cells and blood cells and its clinical significance

Objective To discuss the platinum related excision repair cross-complementation group 1 （ERCC1） mRNA ex- pression in different histological types of lung cancer and its corresponding blood ceils, and to explore the clinical significance, neth- otis Real-time quantitative PCR was used to detect ERCC1 mRNA expression of 105 lung cancer cases（73 ad , 22 squa- mous cell carcinoma and 10 small cell carcinoma） from paraffin-embedded tissues and control blood samples, and the different expres- sions of ERCC1 mRNA in different tissue types were analyzed. Results ERCC1 mRNA expressions were 4. 579± 1. 926 and 8. 324 ± 1. 280 in 105 paraffin-embedded tissues and the corresponding blood samples, showing significant differences（P 〈0. 05 ）. In 105 lung cancer specimens, ERCC1 mRNA expressions in lung squamous cell carcinoma, adenocarcinoma and small cell carcinoma from paraf- fin-embedded tumor cells were 4. 366 ± 1. 822, 4. 724 ± 1. 434 and 4. 366 ± 1. 822, with no difference among the three histological types of lung cancer. The expressions in blood cells were 8. 146 ± 1. 501,8. 336 ± 1. 928 and 8. 664 ± 1. 848, without significance a- mong the three types. ERCC1 mRNA expression was significant differences between paraffin-embedded tumor cells and corresponding blood cells in all the three tissue types（P 〈0. 05）. The expressions had no correlation in tumor cells and blood cells（r =0. 329, P 〉 0. 05 ）. Conclusion Histological types of lung cancer cannot guide platinum-based chemotherapy, and further individual ERCC1 mR- NA detection is needed. Using blood instead of the tumor tissues in individual detection of ERCC1 gene is not advised.