| Axl和Tyro-3受体表达受神经生长因子调控并参与PC12细胞的分化 |
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| 引用本文： | 王琦,卢清君,肖冰,郑焱,王晓民. Axl和Tyro-3受体表达受神经生长因子调控并参与PC12细胞的分化[J]. 神经科学通报, 2011, 27(1): 15-22. DOI: 10.1007/s12264-011-1042-4 |
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| 作者姓名： | 王琦 卢清君 肖冰 郑焱 王晓民 |
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| 作者单位： | 首都医科大学生理学系教育部神经变性疾病重点实验室 |
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| 基金项目： | supported by the National Basic Research Development Program of China(No.2006CB500700);the National Natural Science Foundation of China(No. 30900421/c090201) |
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| 摘 要： | 目的 Axl和Tyro-3受体在脑内有区域性的分布,但两者在中枢神经系统中的生物学功能尚不明确.本研究旨在探讨Axl和Tyro-3受体在神经元分化中的作用.方法 PC12细胞分别转染CMV-Axl-eGFP、CMV-Tyro-3-eCFP和CMV-eGFP质粒后,给予神经生长因子(nerve growth factor...
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| 关 键 词： | Axl Tyro-3 神经生长因子 PC12细胞 分化 |
| 收稿时间： | 2010-11-08 |
Expressions of Axl and Tyro-3 receptors are under regulation of nerve growth factor and are involved in differentiation of PC12 cells |
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| Qi Wang,Qing-Jun Lu,Bing Xiao,Yan Zheng,Xiao-Min Wang. Expressions of Axl and Tyro-3 receptors are under regulation of nerve growth factor and are involved in differentiation of PC12 cells[J]. Neuroscience Bulletin, 2011, 27(1): 15-22. DOI: 10.1007/s12264-011-1042-4 |
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| Authors: | Qi Wang Qing-Jun Lu Bing Xiao Yan Zheng Xiao-Min Wang |
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| Affiliation: | 1. Department of Physiology, Capital Medical University, Key Laboratory for Neurodegenerative Disorders of the Ministry of Education, Beijing, 100069, China
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| Abstract: | Objective Tyro-3 and Axl receptors are expressed in brain in a region-specific manner and their bioactivities in the central nervous system remain still elusive. The aim of the present study was to investigate their functions in neuronal differentiation. Methods PC12 cells overexpressing Tyro-3 or Axl were established by transfection with full-length CMV-Tyro-3-eCFP or CMV-Axl-eGFP plasmid, respectively. CMV-eGFP plasmid served as a control vector. After that, the fluorescence intensity and distributions of green fluorescent protein (GFP) and cyan fluorescent protein (CFP) in the cells with or without nerve growth factor (NGF) treatment were real-time monitored. Results Expressions of Tyro-3 and Axl receptors were under the regulation of NGF and associated with neuronal differentiation. This was not observed in CMV-eGFP-transfected PC12 cells. Besides, confocal microscopy revealed that NGF affected intracellular localization of full-length Axl-eGFP and Tyro-3-eCFP in PC12 cells. Moreover, the development of outgrowth of differentiated PC12 cells under stimulation of NGF was promoted by overexpression of Tyro-3 or Axl. Conclusion Expressions of Tyro-3 and Axl receptors are under the regulation of NGF and are involved in NGF-induced neuronal differentiation of PC12 cells. |
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| Keywords: | Axl Tyro-3 nerve growth factor PC12 cells differentiation |
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