Androgen receptor expression in relation to apoptosis and the expression of cell cycle related proteins in prostate cancer |
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Authors: | Email author" target="_blank">Zahra?AmirghofranEmail author Ahmad?Monabati Naser?Gholijani |
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Institution: | (1) Department of Immunology, Medical School, Shiraz University of Medical Science, Shiraz, Iran;(2) Department of Pathology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran |
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Abstract: | The expression of several genes involved in the regulation of cell cycle and apoptosis may be regulated via the androgen receptor
(AR) in the prostate. AR may have a role in the prognosis of prostatic carcinoma. The aim was to examine AR expression status
and its relationship with markers of proliferation, apoptosis and cell cycle control in prostate cancer. Expression of AR,
bcl-2, bax, Ki-67 and p53 was examined in paraffin-embedded tissues from 50 cases of prostate carcinoma by immunohistochemistry
and evaluated using an index of staining. Detection of apoptotic cells was performed by TUNEL method. Correlation between
AR expression and apoptosis, proliferation index, bcl-2, bax and p53 and also clinicopathological parameters including stage,
pathological grade and Gleason score were determined. AR expression was observed in all cases with mean expression of 81%±15
and mean staining index of 141±65. No correlation was found between AR expression and apoptosis detected in patients. The
mean AR staining index was 170±72 in bcl-2 positive tumors versus 120±53 in bcl-2 negative tumors showing a significant association
between AR and bcl-2 expression (p=0.015). AR expression also showed a significant association with bcl-2/bax ratio (r=0.321,
p=0.023) and Ki-67 proliferation staining index (r=0.396, p=0.004). Although a significant correlation between Ki-67 and p53
with differentiation status of the tumors was observed (p<0.004) no correlation was found with AR. AR expression showed no
prognostic value regarding its correlation with stage and differentiation status of the prostate carcinoma. However, its significant
correlation with Ki-67 and bcl-2 that are markers of cell survival suggest its contribution to tumor cell progression. |
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Keywords: | prostate carcinoma androgen receptor apoptosis p53 bcl-2 bax Ki-67 |
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