安徽省马鞍山市细菌性痢疾监测与耐药性分析

目的 了解马鞍山市引发痢疾的志贺菌优势菌型及其药物敏感状况,为痢疾临床治疗和预防控制提供依据.方法 采用血清凝集试验、菌种ATB鉴定仪系统生化鉴定,和MIC法(最小敏感浓度)对从腹泻患者分离的志贺菌进行药物敏感试验.结果 452份病例样本实验室培养阳性病例67份,其中福氏志贺菌48株,宋内志贺菌19株.马鞍山市流行的优势菌株福氏菌株(B群)48株,有5个血清学亚型和1株不定型,分布如下:F4c 37株;Fla 5株;F2a 2株;F2b 1株;X变种2株.本次监测药敏试验按MIC法进行,药敏试验除氨苄西林(AMP)和复方新诺明(SMZ)耐药以外,其他抗生素:阿莫西林(AMC),头孢噻吩(CFT),头孢噻肟(CTX),庆大霉素(GEN),环丙沙星(CIP),四环素(TBT),利福平(RFA)均敏感.结论 马鞍山市志贺菌感染的优势菌型为福氏4c型,而新型的志贺菌福氏4c型和宋内志贺菌今后应引起足够重视.

Surveillance on bacterial dysentery in the City of Maanshan and analysis on its drug resistance

Objective The purpose of this investigation was to have a better understanding of the predominating strains of shigellae that caused dysentery in the City of Maanshan and their sensitivity to different drugs in order to provide a scientific basis for the prevention and control of the disease. Methods Serum agglutination test together with the biochemical identification with the ATB identification system and minimum inhibitory concentrations (MIC) assay was used for the identification of bacteria and drug sensitivity test on shigellae isolated from patients with diarrhea. Results Of the 452 samples from the patients, there were 67 samples found to be positive, including 48 strains of Sh. Flexneri and 19 strains of Sh. Sonnet. The 48 prevailing Sh. Flexneri strains (B group) prevalent in the City of Maanshan, including 5 strains of 1a, 2 strains of 2a,1 strain of 2b, 37 strains of 4c, 2 strains of X variant, involved 5 serological types with 1 strain unidentified. Drug sensitivity tests by MIC assay revealed that all shigellae were sensitive to ampicillin (AMC), cefalotin (CFT), cefotaxime (CTX), gentamicin (GEN), ciprofloxacin (CIP), tetracycline (TBT), rifampicin (RFA) except ampicillin (AMP) and sulfamethoxazole (SMZ). Conclusion It is seen that Sh. Flexneri 4c is the prevailing strain of shigellae in Maanshan City, at the same time the new Sh. Flexneri 4c and Sh. Sonnet should be taken seriously in the future.