Extrachromosomal genetics of Cephalosporium acremonium |
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Authors: | Paul Tudzynski Karl Esser |
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Institution: | (1) Lehrstuhl für Allgemeine Botanik, Ruhr-Universität, Postfach 102148, D-4630 Bochum 1, Germany |
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Abstract: | Summary In order to facilitate strain improvement by concerted breeding in the cephalosporin producing imperfect filamentous fungus Cephalo sporium acremonium, it is attempted to develop a eukaryotic vector for molecular cloning based on mitochondrial (mt) DNA.Fragments of mtDNA from C. acremonium were inserted into a yeast/bacterial hybrid plasmid (pDAM1) lacking a eukaryotic replicon. Six hybrid plasmids were obtained each containing a different mt fragment which together comprise about 60% of the total mtDNA. One of these recombinant plasmid vectors (pCP2) showed a high replication efficiency which is comparable to that of vectors containing yeast 2 m DNA. This plasmid therefore fulfills the requirements for practical application. |
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Keywords: | Cephalosporium acremonium Eukaryotic Hybrid vector mtDNA |
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