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| 8-Br-cAMP和槲皮素对Eca-109细胞相关基因的DNA拷贝和基因表达的影响 | |
| 引用本文： | 任伟宏,宫璀璀,吴景兰,顾侦芳,朱皖晋,苏安英. 8-Br-cAMP和槲皮素对Eca-109细胞相关基因的DNA拷贝和基因表达的影响[J]. 郑州大学学报(医学版), 2003, 38(5): 675-678 |
| 作者姓名： | 任伟宏 宫璀璀 吴景兰 顾侦芳 朱皖晋 苏安英 |
| 作者单位： | 1. 河南中医学院第一附属医院检验科,郑州,450000 2. 解放军第153医院检验中心,郑州,450042 3. 郑州大学分子细胞生物学研究中心,郑州,450052 4. 河北工程学院医学部,邯郸,056029 |
| 基金项目： | 河南省自然科学基金资助项目 984021800;河南省教育科研基金资助项目 200018007 |
| 摘 要： | 目的探讨8-Br-cAMP和槲皮素对Eca-109细胞wtp53、c-myc、bcl-2基因的扩增、表达以及VEGF表达的影响.方法将贴壁培养的Eca-109细胞随机分成3组,Br组加8-Br-cAMP至终浓度为2×105 mol/L;Q组加槲皮素至终浓度为43 μmol/L;C组不加任何药物,只加入新培养液培养.3组细胞同时培养48h.以cDNA 阵列技术显示Eca-109细胞凋亡中wtp53、c-myc、bcl-2 DNA拷贝和mRNA的表达,以免疫组化法显示VEGF的表达.结果Br组和Q组与C组相比wtp53 DNA拷贝和mRNA信号较强,c-myc、bcl-2 DNA拷贝和mRNA信号较弱,VEGF表达较弱.结论8-Br-cAMP和槲皮素皆可通过上调wtp53,下调bcl-2、c-myc基因的扩增和表达,下调VEGF的表达,而诱导Eca-109细胞凋亡. |
| 关 键 词： | wtp53 c-myc bcl-2 VEGF 细胞凋亡 Eca-109细胞 8-Br-cAMP 槲皮素 食管癌 |
| 修稿时间： | 2003-02-20 |
Expression of Eca-109 cell related genes in cell apoptosis induced by 8-Br-cAMP and quercetin |
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| REN Weihong ),GONG Cuicui ),WU Jinglan ),GU Zhenfang ),ZHU Wanjin ),SU Anying ) ). Expression of Eca-109 cell related genes in cell apoptosis induced by 8-Br-cAMP and quercetin[J]. Journal of Zhengzhou University: Med Sci, 2003, 38(5): 675-678 | |
| Authors: | REN Weihong ) GONG Cuicui ) WU Jinglan ) GU Zhenfang ) ZHU Wanjin ) SU Anying ) ) |
| Affiliation: | REN Weihong 1),GONG Cuicui 2),WU Jinglan 3),GU Zhenfang 1),ZHU Wanjin 1),SU Anying 4) 1)Department of Clinical Laboratory,the First Affiliated Hospital,Henan Traditional Chinese Medicine College,Zhengzhou 450000 2)Laboratory Medicine Center,the PLA No.153 Hospital,Zhengzhou 450042 3)Molecular Cell Biology Research Center,Zhengzhou University,Zhengzhou 450052 4)Medical Center,Hebei Engineering College,Handan 056029 quercetin |
| Abstract: | Aim: To study the gene copies and expression of wtp53, c myc, bcl 2 and expression of VEGF in Eca 109 cell apoptosis. Methods: The cultured Eca 109 cells were randomly divided into 3 groups: 8 Br cAMP (Br)group, Quercetin (Q)group, and Control (C)group with no any drugs. The 3 group cells were cultured simultaneously for 48 h. The expression and copies of wtp53, c myc , and bcl 2 gene were demonstrated by cDNA array and expression of VEGF was evaluated by immunohistochemistry. Results:Compared with the C group, the copies and mRNA expression of wtp53 gene in Br and Q groups were enhanced, while that of c myc, bcl 2 genes were decreased;the expression of VEGF was also attenuated.Conclusion:8 Br cAMP and quercetin could induce Eca 109 cell apoptosis through up regulating gene copies and expression of wtp53 and down regulating that of bcl 2 and c myc as well as down regulating the expression of VEGF. |
| Keywords: | wtp53 c myc bcl 2 VEGF apoptosis Eca 109 cell 8 Br cAMP |
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