Chloride adduct tandem mass spectrometry for the quantification of thiamphenicol and florfenicol in bovine muscle期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Chloride adduct tandem mass spectrometry for the quantification of thiamphenicol and florfenicol in bovine muscle

Affiliation:	1. University of Botswana, Faculty of Science, Department of Chemistry, PB UB 00704, Gaborone, Botswana;2. The National Veterinary Laboratory, PB0035, Gaborone, Botswana;1. The George Institute for Global Health, Sydney, NSW, 2042, Australia;2. Charles Perkins Centre and School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, 2006, Australia;3. Department of Epidemiology and Biostatistics, School of Public Health, Faculty of Medicine, Imperial College London, United Kingdom;1. Department of Chemistry, Wuhan University, Wuhan 430072, PR China;2. Hubei Provincial Institute for Food Supervision and Test, Wuhan 430075, PR China;3. HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan;4. Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan 430072, PR China;1. Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Olsztyn, Poland;2. Department of Plant Breeding and Seed Production, University of Warmia and Mazury, Olsztyn, Poland;3. Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn, Poland;4. Department of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury, Olsztyn, Poland;1. Livestock and Wildlife Laboratory, Arid Lands Institute, Medenine, Tunisia;2. Faculty of Sciences of Gabès, University of Gabès, Tunisia;3. Department of Bioactivity and Food Analysis, Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CIAL), CSIC-UAM, Autónoma University of Madrid, Spain;1. CONICET- Universidad De Buenos Aires, Instituto de Investigaciones en Producción Animal, (INPA), CP C1427CWO, Buenos Aires, Argentina;2. Universidad Nacional del Litoral, Facultad de Ingeniería Química Programa De Investigación y Análisis de Residuos y Contaminantes Químicos (PRINARC), Santiago del Estero 2654 Piso 5-6, CP 3000 Santa Fe, Argentina;3. Universidade Federal do Paraná (UFPR), Pós-Graduação em Engenharia Ambiental, Departamento de Engenharia, CP 19020, Bairro Jardim Das Américas, Curitiba, Paraná, Brazil

Abstract:	LC–MS/MS analytical performance characteristics of chloride adduct ions [M + Cl]⁻ of thiamphenicol (TAP) and florfenicol (FFC) as precursor ions for quantification were studied. The calculations were based on a THF/Water solvent system to which dichloromethane was added as an additive at a composition of 2 % to generate chloride (Cl⁻) ions during electrospray ionization (ESI) of the solutions. The selectivity and signal to noise (S/N) ratio when the [M + Cl]⁻ adduct ions were used as precursor ions were excellent compared to deprotonated [M−H]⁻ ions that are traditionally used to determine the two analytes. The S/N ratios in the total ion chromatogram (TIC) of TAP and FFC were 577 and 3062 respectively, compared to 167 and 452 when [M−H]⁻ were used at the same spike levels of 50 and 100 μg/kg respectively. The limits of detection (LODs) for TAP and FFC were 4.0 and 3.7 μg/kg respectively using [M + Cl]⁻ as opposed to 2.1 and 4.3 μg/kg respectively when [M−H]⁻ were used. [M + Cl]⁻ adduct ions proved to be excellent substitutes for [M−H]⁻ especially where identity and selectivity would be the key drivers of the analytical method before quantification of the two analytes.

Keywords:	Florfenicol Thiamphenicol Food analysis Food composition Adduct ions LC–MS/MS Electrospray Chloride
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