Isolation and determination of lipophilic mycochemicals from a New Zealand edible native mushroom Hericium novae-zealandiae |
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| Institution: | 1. Food Science, School of Chemical Sciences, The University of Auckland, Auckland 1010, New Zealand;2. Center for Drug Research and Development, Guangdong Pharmaceutical University 510006, Guangzhou, China;3. Manaaki Whenua - Landcare Research, Auckland 1072, New Zealand;4. Riddet Institute, New Zealand Centre of Research Excellence for Food Research, Palmerston North 4474, New Zealand;1. ICAR-Indian Agricultural Research Institute (IARI), New Delhi, India;2. Regional Research Centre-IARI, Dharwad, Karnataka, India;3. CSK-Himachal Pradesh Krishi Vishvavidyalaya, Bajaura Centre, Himachal Pradesh, India;4. ICAR-Indian Institute of Maize Research (IIMR), Ludhiana, Punjab, India;1. Key Laboratory of Economic Plants and Biotechnology/Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;2. Dr. Plant Skin Care Products Co - Development Center, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China;3. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, 650201, China;1. College of Life Sciences, Shanghai Normal University, Shanghai, 201418, PR China;2. College of Chemistry and Chemical Engineering, Anhui University of Technology, Ma’anshan, 243002, PR China;3. Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, 830011, PR China;1. Department of Cosmetic Science, Chang Gung University of Science and Technology, Guishan, Taoyuan, 333, Taiwan, ROC;2. Research Center for Food and Cosmetic Safety and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Guishan, Taoyuan, 333, Taiwan, ROC;3. Department of Anesthesiology, Chang Gung Memorial Hospital, Guishan, Taoyuan, 333, Taiwan, ROC;4. National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, 112, Taiwan, ROC;5. Biotechnology Center, Grape King Bio Ltd., Chung-Li, Taoyuan, 320, Taiwan, ROC;6. Institute of Biopharmaceutical Science, National Yang-Ming University, Taipei, 112, Taiwan, ROC;1. School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea;2. College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea;3. College of Pharmacy, Wonkwang University, Iksan 570-749, Republic of Korea;1. Research Institute of Biotechnology, Hungkuang University, 34 Chung-Chie Rd., Shalu County, Taichung City 43022, Taiwan;2. Division of General Education, Hungkuang University, 34 Chung-Chie Rd., Shalu County, Taichung City 43022, Taiwan;3. Department of Pharmacy, Kuan-Tien General Hospital, Shalu County, Taichung City 43302, Taiwan;4. Graduate Institute of Clinical Medicine, Taipei Medical University, 250 Wu-Xing St., Taipei 10031, Taiwan;5. Research Institute of Medical Sciences, Taipei Medical University, 250 Wu-Xing St., Taipei 10031, Taiwan;6. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, 252, Wu-Hsing St., Taipei 11031, Taiwan;7. Divison of Gastroenterology and Hepatology, Department of Internal Medicine, School of Medicine, Taipei Medical University, 250, Wu-Hsing St., Taipei 11031, Taiwan |
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| Abstract: | Hericium novae-zealandiae is a native mushroom consumed by indigenous Māori people in New Zealand. The lipophilic mycochemicals of the mushroom were isolated using a normal column chromatography combined with a preparative HPLC. Structural characterisation based on spectroscopic methods, namely UV, MS, NMR and single crystal XRD have identified three lipophilic compounds as hericene B (a compound unique to Hericium), ergosterol and ergosterol peroxide. Following this, an HPLC-DAD method was developed and validated to quantify the hericene B and ergosterol. The method showed excellent selectivity, linearity, precision, accuracy and robustness. The content of hericene B was determined as 28.53 mg/g by dry weight of H. novae-zealandiae (approximately 3%). This discovery indicates the potential utilisation of H. novae-zealandiae as a natural source of hericene B. Current research revealed for the first time, the lipophilic constituents of H. novae-zealandiae and the method development for quantification of hericene B in the above species. |
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| Keywords: | Mushroom Mycochemicals Hericene B Ergosterol Ergosterol peroxide HPLC Column chromatography Spectroscopy NMR Single crystal X-ray diffraction Food composition Food analysis |
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