Use of Quick,Easy, Cheap,Effective, Rugged & Safe (QuEChERS) and molecular imprinted polymer followed by gas chromatography with tandem mass spectrometry for the quantitative analysis of polycyclic aromatic hydrocarbons (PAH4) in complex health supplements |
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| Affiliation: | 1. Analytical Services Department, Biosearch S.A. Camino de Purchil 66, E-18004 Granada, Spain;2. Analytical Chemistry and Life Sciences Research Group, Analytical Chemistry Department, University of Granada, Avda. Fuentenueva s/n, E-18071 Granada, Spain;1. School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, China;2. School of Chemistry and Materials Science, Northwest University, Xi''an 710127, China;1. State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China;2. School of Environmental Studies, China University of Geosciences, Wuhan 430074, China;1. Centre d’expertise en analyse environnementale du Québec, ministère du Développement durable, de l’Environnement et de la Lutte contre les changements climatiques, Québec, Canada;2. Institut national de la recherche scientifique – Institut Armand-Frappier, Laval, Canada |
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| Abstract: | A gas chromatography-tandem mass spectrometry sensitive and selective method based on a combination of QuEChERS and subsequent molecularly imprinted polymer (MIP) technology was validated for the analysis of polycyclic aromatic hydrocarbons in complex dry extracts of Eleutherococcus senticosus, Salvia officinalis, Camellia sinensis, Zingiber officinale, Uncaria tomentosa, Humulus lupulus, Pinus sylvestris L., Spirulina maxima, propolis and royal jelly. The method has been optimized using gas chromatography coupled to tandem mass spectrometry. An additional sample treatment of the dry extracts, based on the combined use of MIP-SPE and QuEChERS, was required because of the strong matrix effect observed related to interferences affecting analyte quantification. Estimation of the method detection limit and quantification limit was carried out for validation. The LOQs were 0.2 ng g−1 for benz[a]anthracene, 0.3 ng g−1 for benzo[a]pyrene and 0.4 ng g−1 for benzo[b]fluoranthene and chrysene; and the LODs were 0.07, 0.09 and 0.1 ng g−1 respectively. Recoveries were in the range of 88.5 % for B[b]F, to 114 % for B[a]A, and % RSD was < 14 % in all cases. The method was applied in routine analysis to a wide variety of dry extracts from EU and non-EU manufacturers. |
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| Keywords: | Food supplements Health supplements Dispersive solid phase extraction (QuEChERS) Molecularly imprinted polymer (MIP) GC–MS/MS |
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