Application of recombinase polymerase amplification method for rapid detection of infectious laryngotracheitis virus |
| |
| Institution: | 1. Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang 050051, China;2. College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China;3. Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China;4. Hebei Animal Disease Control Center, Shijiazhuang 050050, China;5. Center of Inspection and Quarantine Technology, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang 050051, China;1. Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals, Monitoring Institute, Guangzhou, China;2. School of Science, RMIT University, Melbourne, Australia |
| |
| Abstract: | Infectious laryngotracheitis is a significant respiratory disease of chickens that causes huge economic losses due to high morbidity and mortality and reduced egg production. A real-time recombinase polymerase amplification (RPA) assay was developed to accurately detect ILTV. The specific probe and primer sets were carefully designed and screened. The real-time RPA assay was carried out at 39 °C for 30 min, and results were obtained within 15 min. The results of the specificity assay showed no fluorescence signals with other avian-related viruses. The sensitivity of the assay was 1 × 102 copies/μL. The low CV value showed that the assay was reproducible. A total of 115 clinical samples were tested using the real-time RPA assay and the real-time PCR assay in parallel; the coincidence rates of the two detection methods were 100%. The results indicated that the real-time RPA assay is a specific, sensitive, rapid, and useful tool for epidemiological studies and clinical diagnosis, especially in the field and in resource-poor areas. |
| |
| Keywords: | Infectious laryngotracheitis virus Real-time Recombinase polymerase amplification Detection |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
