靶肌肉注射促红细胞生成素对大鼠周围神经再生的作用

目的探讨靶肌肉注射人重组促红细胞生成素（recombinant human erythropoietin，rh-EP0）对大鼠坐骨神经损伤后神经再生的作用。方法选用健康雄性SD大鼠12只，制备大鼠右侧坐骨神经钳夹损伤模型。实验动物随机分为2组，每组6只，EPO组：靶肌肉注射rh-EPO2500U／kg；对照组：注射同体积的生理盐水。术后第7d、14d、21d观察坐骨神经功能指数（SFI），第21d组织学观察脊髓腰膨大（L4～L6）、夹伤远端坐骨神经、损伤侧腓肠肌组织并作图象分析测定脊髓前角运动神经元数、再生有髓神经纤维数、髓鞘厚度、轴突直径和腓肠肌肌细胞横截面积等指标。结果术后第7d两组SFI无显著性差异，术后第14d、21dEPO组SFI恢复程度明显大于对照组，差别有显著性意义（P〈0．05）；术后第21d损伤侧脊髓前角运动神经元数、再生有髓神经纤维数、髓鞘厚度、轴突直径和腓肠肌肌细胞横截面积等指标，EPO组均优于对照组（P〈0．01，P〈0．05）。结论靶肌肉注射rh-EPO能促进周围神经再生和功能恢复。

Effect of target muscle injection of recombinant human erythropoietin on peripheral nerve regeneration in rats

Objective To investigate the effect of target muscle injection of recombinant human erythropoietin（rh-EPO） on the nerve regeneration of adult rats sciatic nerves. Methods 12 healthy male Sprague-Dawley rats were involved and right sciatic nerve crushed model was used.The experimental rats were divided randomly into two groups： the EPO group and the control group, 6 rats in each group, rh-EPO 2500U/kg was injected daily into the gastrocnemius in EPO gro- up, and normal saline was injected into the gastrocnemius every day after operation in control group. At days 7,14 and 21 after operation, the sciatic function index （SFI） was determined by walking tract analysis. At 21 days after operation, the spinal cord segments （IA-L6）,the distal site of injured sciatic nerve and the injured gastrocnemius muscle were observed by light microscope.The count of motor neurons of anterior horn, the number of myelinated fibers, the cross section areas of gastrocnemius muscle cells and other parameters were determined. Results At day 7 after operation, SFI scores had no significant difference between two groups;at day 14 and 21 after operation ,SFI score of EPO group was significantly higher than that of the control group（P〈0.01）. At day 21 after operation,the numbers of motor neurons of anterior horn, myelinated nerve fibers,diameter of axon,thick of myelin sheath and the cross section areas of gastrocnemius muscle cells in EPO group were significantly superior to those in control group（P〈0.05 ,P〈0.01）. Conclusion Target muscle injected rh-EPO can promote the injured nerve regeneration and improve the recovery of their function.