| 碱性成纤维细胞生长因子对骨髓间充质干细胞向颞下颌关节盘细胞分化的影响 |
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| 引用本文： | 苏雪莲,包广洁,康宏.碱性成纤维细胞生长因子对骨髓间充质干细胞向颞下颌关节盘细胞分化的影响[J].生物医学工程学杂志,2012(4):732-736. |
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| 作者姓名： | 苏雪莲 包广洁 康宏 |
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| 作者单位： | 西北民族大学国家民委口腔医学重点实验室;兰州大学口腔医学院 |
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| 基金项目： | 甘肃省国际合作项目资助(0804WCGA127);甘肃省科技支撑计划项目资助(1104FKCA144)Δ |
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| 摘 要： | 为观察碱性成纤维细胞生长因子(bFGF)对骨髓间充质干细胞(BMSCs)分化的影响,采用bFGF诱导山羊BMSCs,通过组织学、免疫组织化学和酶联免疫吸附实验(ELISA)等方法检测BMSCs产生的细胞外基质(ECM)包括Ⅰ型胶原和蛋白多糖的情况,初步评价BMSCs做为颞下颌关节(TMJ)盘组织工程种子细胞的可行性。结果表明:经bFGF诱导,BMSCs向成纤维细胞样细胞分化,能够合成糖胺聚糖(GAG)和I型胶原,因此认为BMSCs作为工程化关节盘种子细胞是可行的。
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| 关 键 词： | 碱性成纤维细胞生长因子 骨髓间充质干细胞 颞下颌关节盘 组织工程 |
Effects of Basic Fibroblast Growth Factor on Bone Marrow Mesenchymal Stem Cell Differentiation into Temporomandibular Joint Disc Cells |
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| Su Xuelian,Bao Guangjie,Kang Hong.Effects of Basic Fibroblast Growth Factor on Bone Marrow Mesenchymal Stem Cell Differentiation into Temporomandibular Joint Disc Cells[J].Journal of Biomedical Engineering,2012(4):732-736. |
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| Authors: | Su Xuelian Bao Guangjie Kang Hong |
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| Institution: | 1(Key Lab of Stomatology of State Ethnic Affairs Commission,Medical College,Northwest University for Nationalities,Lanzhou 730030,China) 2(School of Stomatology,Lanzhou University,Lanzhou 730000,China) |
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| Abstract: | The present paper is aimed to observe the effects of basic fibroblast growth factor(bFGF) on bone marrow mesenchymal stem cell(BMSCs) differentiation.The bFGF was used to stimulate BMSCs and histology,immunohistochemistry and enzyme linked immunosorbent assay(ELISA) were used to examine the extracellular matrix produced by induced BMSCs,evaluated the feasibility of BMSCs being the seeding cells of temporomandibular joint(TMJ) disc tissue engineering.The results showed that having been induced with bFGF,the BMSCs could differentiate into fibroblast-like cells,which could synthesize GAG and collagen type I matrix.So it is feasible for BMSCs as seeding cells for engineered TMJ disc. |
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| Keywords: | Basic fibroblast growth fator(bFGF) Bone marrow mesenchymal stem cells(BMSCs) Temporomandibular joint(TMJ) disc Tissue engineering |
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