新疆喀什地区利什曼原虫人群感染率调查

目的了解现阶段新疆喀什地区人群利什曼原虫感染现状，为制定防治规划提供依据。比较PCR和ELISA检测利什曼原虫无症状感染的效能。方法选取2009年9月新疆喀什地区黑热病流行严重的1县3乡四个调查点，在当地疾病预防控制中心的帮助下，采集黑热病患者及其家属和邻居的血液样品，分别编号，每份血样分抗凝血和非抗凝血。采用ELISA方法检测血清中利什曼原虫特异性抗体；用两组PCR引物RV1、RV2和K13A、K13B检测血样中利什曼原虫特异DNA片段。结果PCR法阳性检出率为82．67％，ELISA法阳性检出率为69．33％。结论现阶段新疆喀什地区黑热病感染率仍然颇高，尤其表现在与患者密切接触的的患者家属和邻居；PCR是检测无症状感染较敏感的方法。

Survey of Leishmani donivani infection in humans in Kashi City of Xinjiang Uggur Autonomous Region

Aim To understand current situation of Leishnmnia Donovani infection in Kashi City of XinJiang Province. Methods Blood samples were coUeceted from Kala azar patients, family members of the patients and the patients＇ neighbours during September, 2009 from one county and three townships in Kashi City of Xinjiang Uggur Autonomous Region with the help of local CDC.The blood samples sorted as anticoagulation and non-anticoagulation. Antibodies against Leishmania donovani were determined by ELISA; the leishmania-specific DNA in venous blood samples were detected by means of PCR assay with two pairs of primers RVI-RV2 and K13A-K13B. Results The positive rate of blood samples detected by PCR was 82.67%,those detected by ELISA was 69.33%. Conclusion The prevalence of Leishmania in human body is rather high and the work of monitoring of Leishimania donivani infection be strengthened and PCR is a sensitive assay for detection of asymptomatic infections in human.