| 抑制自分泌或旁分泌骨形成蛋白和血小板源性生长因子信号可实现大鼠神经干细胞的长期增殖 |
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| 引用本文： | 孙一睿,周良辅,吴惺,袁强,毛颖,胡锦.抑制自分泌或旁分泌骨形成蛋白和血小板源性生长因子信号可实现大鼠神经干细胞的长期增殖[J].中国神经再生研究,2011,6(13):965-971. |
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| 作者姓名： | 孙一睿 周良辅 吴惺 袁强 毛颖 胡锦 |
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| 作者单位： | 复旦大学上海华山医院神经外科，上海 200040；上海交通大学附属上海市第六人民医院，上海 200233,复旦大学上海华山医院神经外科，上海 200040,复旦大学上海华山医院神经外科，上海 200040,上海交通大学附属上海市第六人民医院，上海 200233,复旦大学上海华山医院神经外科，上海 200040,复旦大学上海华山医院神经外科，上海 200040 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目）;中国博士后科学基金;国家教育部回国人员科研启动基金 |
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| 摘 要： | Continuous expansion of rat neural stem cell lines has not been achieved due to proliferation arrest and spontaneous differentiation in vitro. In the current study, neural precursor cells derived from the subventricular zone of adult rats spontaneously underwent astroglial and oligodendroglial differentiation after limited propagation. This differentiation was largely induced by autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signals. The results showed that, by inhibiting bone morphogenetic protein and platelet derived growth factor signals, adult rat neural precursor cells could be extensively cultured in vitro as tripotent stem cell lines. In addition to adult rat neural stem cells, we found that bone morphogenetic protein antagonists can promote the proliferation of human neural stem cells. Therefore, the present findings illustrated the role of autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signaling in determining neural stem cell self-renewal and differentiation. By antagonizing both signals, the long-term propagation of rat neural stem cell lines can be achieved.
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| 关 键 词： | neural stem cells cell dormancy proliferation arrest stem cell lines bone morphogenetic protein platelet derived growth factor |
Prolonged propagation of rat neural stem cells relies on inhibiting autocrine/paracrine bone morphogenetic protein and platelet derived growth factor signals |
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| Yirui Sun,Liangfu Zhou,Xing Wu,Qiang Yuan,Ying Mao and Jin Hu.Prolonged propagation of rat neural stem cells relies on inhibiting autocrine/paracrine bone morphogenetic protein and platelet derived growth factor signals[J].Neural Regeneration Research,2011,6(13):965-971. |
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| Authors: | Yirui Sun Liangfu Zhou Xing Wu Qiang Yuan Ying Mao and Jin Hu |
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| Institution: | 1Department of Neurosurgery, Shanghai Huashan Hospital, Fudan University, Shanghai 200040, China 2Shanghai No. 6 People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China |
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| Abstract: | Continuous expansion of rat neural stem cell lines has not been achieved due to proliferation arrest and spontaneous differentiation in vitro. In the current study, neural precursor cells derived from the subventricular zone of adult rats spontaneously underwent astroglial and oligodendroglial differentiation after limited propagation. This differentiation was largely induced by autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signals. The results showed that, by inhibiting bone morphogenetic protein and platelet derived growth factor signals, adult rat neural precursor cells could be extensively cultured in vitro as tripotent stem cell lines. In addition to adult rat neural stem cells, we found that bone morphogenetic protein antagonists can promote the proliferation of human neural stem cells. Therefore, the present findings illustrated the role of autocrine or paracrine bone morphogenetic protein and platelet derived growth factor signaling in determining neural stem cell self-renewal and differentiation. By antagonizing both signals, the long-term propagation of rat neural stem cell lines can be achieved.
Key Words: neural stem cells; cell dormancy; proliferation arrest; stem cell lines; bone morphogenetic protein; platelet derived growth factor |
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| Keywords: | rat neural stem cells bone morphogenetic protein platelet derived growth factor propagation |
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