广东省脑膜炎奈瑟氏菌porA基因限制性片段长度多态性分型研究

目的 分析广东省在不同流行时期，特别是80年代后期以来脑膜炎奈瑟氏菌（Nm)的特点。方法 选择35株A、B、C群流脑菌株（1966－2000年）进行porA基因聚合酶链反应－限制性片段长度多态性（PCR－RFLP)分型研究，采用PCR反应扩增porA基因，PCR产物纯化后经限制性内切酶MspI酶切；10％聚丙烯酰胺凝胶（PAGE）电泳分离酶切产物，银染显色。结果 35株被检菌株分为5种RFLP型，20株A群菌株（病人17株，带菌3株）分3型，谱型与我国代表谱型相同（即a1、a2、a3型）；11株B群菌株分5型，其中6株病人菌株每两株1型，分为3型（即b20、b21、b21a2型），其中b20型与a3型谱型相同；4株C群菌株有3株与b21a2菌型相同。结论 不同的血清群可有相同的RFLP谱型；A群Nm不同的流行周期有自己优势菌型，广东省60年代与80年代后期至今的代表菌型相同，为a3型；B群RFLP谱型比A群变异程度大，同一时期分离到的菌株可有几种不同的RFLP谱型；广东省流脑流行存在由A群向B群变迁的趋势。

Study on PCR-RFLP Patterns of porA Gene of Neisseria Meningidis in Guangdong Province

Objective To determine the characteristics of Neisseria meningitides (Nm) in Guangdong Province in different epidemic periods, especially after late 1980s. Methods A total number of 35 Nm strains of serogroups A, B, and C isolated from 1966 to 2000 in Guangdong Province were typed by porA genes amplified with PCR, digested with restriction endonuclease Msp I, seperated on 10% polyacrylamide gels and stained with silver. Results\ 35 Nm strains were divided into 5 distinct patterns. 20 strains of serogroup A were divided into 3 distinct patterns (a 1, a 2 and a 3), similar to the predominant of China. 11 strains of serogroup B were divided into 5 distinct patterns, of which 6 strains presented 3 patterns (b 20 , b 21 , b 21 a 2). 3 of 4 strains of serogroup C had b 21 a 2 pattern. Conclusion Nm strains of different serogroups can have the same RFLP patterns. In different epidemic periods, serogroup A was predominant. In 1960s and from the late 1980s to 2000, Strains of a 3 were the representative. Nm strains of serogroup B had several different RFLP patterns, and were more variable than that of serogroup A. Nm strains in Guangdong Province showed a changing trend from serogroup A to serogroup B.