乌龙茶多糖对糖尿病鼠免疫功能的影响

目的:研究乌龙茶多糖(OTPS)对糖尿病(DM)模型鼠免疫功能的影响。方法:以链脲佐菌素复制Balb/CDM小鼠和WistarDM大鼠模型,依据卫生部保健食品功能学评价程序和检验方法,检测脏器/体重比值、迟发型变态反应(DTH)、半数溶血值(HC50)、腹腔巨噬细胞吞噬功能、自然杀伤细胞(NK)活性,血清IL-2,并用流式细胞仪(FCM)分析外周血免疫分子。结果:(1)OTPS能极显著提高DM小鼠NK细胞的活性,促进T细胞介导的DTH反应,增强巨噬细胞的吞噬功能以及提高血清HC50值,同时还能增加胸腺指数和脾指数。(2)FCM测试表明OTPS能降低DM鼠T淋巴细胞中毒性CD8T淋巴细胞比例,增加CD4T淋巴细胞,减少过度表达的NK细胞,降低活化的T淋巴细胞数量,从而减少IL-2的含量,纠正免疫功能紊乱现象。结论:OTPS对DM模型鼠有较好的免疫恢复功能。

INFLUENCE OF OOLONG TEA POLYSACCHARIDES ON IMMUNOMODULATORY FUNCTION OF DIABETIC MICE AND RATS

Objective: To study the influence of Oolong tea polysaccharides (OTPS) on immunomodulatory function of diabetic mice. Method: The immunomodulatory effects of OTPS on STZ-induced Balb/C diabetic mice were measured by delayed type hypersensitivity (DTH), phagocytosing method, lactodehydrogenase (LDH) method etc. FCM was used to detect the rates of CD in serum of diabetic rats. Results: (1) OTPS could significantly intensify DTH caused by DNFB, enhance the activity of NK cells, improve phagocytotic function of peritoneal macrophage and increase HC50 in serum. Besides, OTPS could raise thymus index and spleen index of diabetes mice. (2) OTPS could decrease the rate of CD8 T cells and increase the percentage of CD4 T cells and the ratio of CD4/CD8 in serum of diabetes rats. Meanwhile, OTPS could also inhibit over expression of NK cells and reduce the rate of activated T cells. Collectively, these data proved that OTPS could correct the imbalance of immunoregulation in diabetic rats in vivo. Conclusion: There was remarkable effect of Oolong tea polysaccharides on immunomodulatory function of diabetic mice and rats.