自身免疫性大疱性皮肤病患者血清中IgM抗体所识别的皮肤抗原的研究

目的:研究自身免疫性大疱性皮肤病患者血清中IgM抗体所识别的皮肤抗原成分.方法:该研究共纳入10例大疱性类天疱疮患者、15例天疱疮患者(包括7例红斑/落叶型和8例寻常型).首先通过直接免疫荧光法分析受试患者皮肤中所沉积的免疫球蛋白或补体,再通过免疫印迹方法分析患者血清中的IgM抗体所识别的皮肤抗原成分.结果:在10例大疱性类天疱疮患者的皮肤中,C3、IgG、IgM单独沉积的例数分别是4例、2例、1例,IgG和C3共同沉积的2例,IgG、C3和IgA三者共同沉积的1例;在15例天疱疮患者中,C3、IgG单独沉积的例数分别是4例和2例,IgG和C3共同沉积的6例,IgM和C3共同沉积的3例.免疫印迹研究发现9例(9/10)大疱性类天疱疮患者、11例(11/15)天疱疮患者血清中的IgM抗体可以识别皮肤中分子量约80 kD的蛋白质.结论:IgM在自身免疫性大疱性皮肤病患者的皮肤中沉积的几率很低,但大多数患者血清中的IgM抗体都能够识别分子量约80 kD的皮肤抗原.

Investigation on the Cutaneous Antigen Recognized by Serum IgM Antibody from Patients with Autoimmune Bullous Diseases

Objective：To investigate on the IgM antibodies from patients with autoimmune pemphigoid （BP） and 15 ones with pemphigus deposit of immunoreactants in skin of patients cutaneous antigens that were recognized by serum bullous diseases, nethods：10 cases with bullous vulgaris/foliaceus were involved in this study. The were detected by direct immunofluorescence. The cutaneous antigens recognized by serum IgM antibodies were detected by Western Blot. Results： The existent pattern of immunoreactants in skin in BP patients were as following （ number of ca- ses ） ： purely C3 （4）, purely IgG （2）, purely IgM （ 1 ）, IgG ＋ C3 （2） and IgG ＋ C3 ＋ IgA （ 1 ）, while the existent pattern in patients with pemphigus were： purely C3 （4）, purely IgG （2）, IgG ＋ C3（6）, IgM ＋ C3 （3）. Serum IgM antibodies from 9 of 10 patients with BP and 11 of 15 ones with pemphigus were found to bind a cutaneous protein of about 80 kD. Conclusion： IgM were rarely detected from the skin of patients with autoimmune bullous diseases. However, serum IgM from the majority of patients recognized a cutaneous protein of about 80 kD.