| 2-(2-苯并呋喃基)-2-咪唑啉对大鼠脑缺血再灌注损伤后Bcl-2和Bax表达的影响 |
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| 引用本文： | 杨金龙,程朝辉,刘双,韩钊,侯圣陶.2-(2-苯并呋喃基)-2-咪唑啉对大鼠脑缺血再灌注损伤后Bcl-2和Bax表达的影响[J].温州医学院学报,2012,42(2):129-131. |
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| 作者姓名： | 杨金龙 程朝辉 刘双 韩钊 侯圣陶 |
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| 作者单位： | 1. 温州医学院附属第一医院脑血管科,浙江温州,325000
2. 温州医学院附属第一医院神经生物研究所,浙江温州,325000 |
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| 基金项目： | 温州市科技局对外合作项目 |
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| 摘 要： | 目的:观察脑缺血再灌注后2-(2-苯并呋喃基)-2-咪唑啉(2-BFI)对凋亡相关蛋白Bcl-2和Bax表达的影响.方法:采用线栓法建立SD大鼠脑缺血再灌注模型,随机分为假手术组、模型组和用药组(2-BFI 3 mg/kg).于再灌注24 h后断头取脑,用免疫组化染色法检测Bcl-2和Bax蛋白的表达.结果:用药组Bcl-2阳性细胞数明显高于模型组(P＜0.01),Bax阳性细胞数明显低于模型组(P＜0.01).结论:2-BFI于脑缺血再灌注后立即给药能显著上调Bcl-2蛋白的表达,下调Bax蛋白的表达.
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| 关 键 词： | 2-(2-苯并呋喃基)-2-咪唑啉 局灶性脑缺血再灌注模型 Bcl-2 Bax 大鼠 |
Effect of 2- (2-benzo fu-ranyl) -2-imidazoline on expression Bcl-2 and Bax in ischemiareperfusion injury rats |
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| YANG Jinlong
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CHENG Zhaohui
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LIU Shuang
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HAN Zhao
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HOU Shengtao.Effect of 2- (2-benzo fu-ranyl) -2-imidazoline on expression Bcl-2 and Bax in ischemiareperfusion injury rats[J].Journal of Wenzhou Medical College,2012,42(2):129-131. |
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| Authors: | YANG Jinlong CHENG Zhaohui LIU Shuang HAN Zhao HOU Shengtao |
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| Institution: | .Division of Stroke Medicine,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou,325000 |
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| Abstract: | Objective: To observe the neuroprotection of 2-(2-benzofuranyl)-2-imidazoline(2-BFI) on the expression of Bcl-2/Bax after cerebral ischemia reperfusion(CIR).Methods: Tran-sient focal ischemia was induced by suture occlusion of the middle cerebral artery in rats and then randomized into sham group,model group,2-BFI treatment group(3 mg/kg).After 24 h of CIR,brain tissues of rats were selected.The expressions of Bcl-2/Bax in brain were examined with immunohistochemistry.Results: Treatment with 2-BFI could significantly im-prove the number of Bcl-2,compared with model rats(P <0.01),reduce the positive cell numbers of Bax compared with vehicle-treated rats(P <0.01,both).Conclusion: 2-BFI can significantly increase the expression of Bcl-2,down-regulates the expression of Bax. |
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| Keywords: | 2-BFI cerebral ischemia reperfusion model Bcl-2 Bax rats |
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