D-半乳糖致衰老小鼠胸腺T细胞重要膜型分子的改变及其意义

目的:建立D-半乳糖亚急性衰老小鼠模型,并探讨其胸腺T细胞重要膜型分子的改变及其意义。方法:8周龄雌性昆明种小鼠,12.5 mL/(kg.d)颈后部皮下注射100 g/LD-半乳糖溶液,连续注射42 d;逐日观察小鼠的生存状态和行为变化,并通过对小鼠血清中SOD活力和MDA含量的检测,对此衰老模型进行生物学鉴定;在D-半乳糖亚急性衰老小鼠模型成功建立的基础上,采用免疫荧光标记技术和流式细胞仪检测技术对胸腺T细胞重要膜型分子进行检测分析。结果:造模过程中小鼠逐渐表现出脊椎隆起,体形消瘦,皮肤松弛等衰老体征;血清中SOD活力下降模型组:(2.16±0.43)mKat/L,对照组:(5.52±1.55)mKat/L,P<0.01],MDA含量上升模型组:(4.14±0.82)μmol/L,对照组:(1.24±0.21)μmol/L,P<0.01];小鼠胸腺初始T细胞相关分子CD45RA表达下降模型组:(2.16±0.47)%,对照组:(2.98±0.53)%,P<0.05];小鼠胸腺T细胞活化相关分子CD28模型组:(91.52±1.68)%,对照组:(95.12±1.21)%,P<0.05]和CD25模型组:(7.42±0.75)%,对照组:(8.84±0.58)%,P<0.05]表达下降;小鼠胸腺T细胞活化负性调控分子PD-1表达上升模型组:(21.25±1.95)%,对照组:(12.92±3.28)%,P<0.01];小鼠胸腺记忆T细胞相关分子CD196表达上升,但与对照组相比没有显著性差异模型组:(21.13±1.44)%,对照组:(19.73±2.02)%]。结论:成功建立了D-半乳糖亚急性衰老小鼠模型,机体衰老时胸腺中初始和活化T细胞减少,记忆T细胞有增加的趋势。

Changes and effects of significant membrane molecules on thymic T cell of aging mice induced by D-galactose

AIM: To establish subacute aging mice model by D-galactose and to explore the changes and effects of significant membrane molecules on thymic T cell.METHODS: Female Kunming mice of 8 weeks old were injected with D-galactose of 12.5 mL/(kg·d) by subcutaneous in scruff for 42 days.The animals’ living conditions and biological behaviors were observed everyday.SOD activities and MDA content of serum were measured to determine whether the aging model was successfully established.On the basis of successfully establishing aging model,detect the significant membrane molecules of thymic T cell by Immunofluorescence technique and Flow Cytometer.RESULTS: During the 42 days,gradually,the model mice showed bending body,loose skin,slow action and so on.The activities of SOD in the serum were significantly decreased(P<0.01),and the content of MDA in the serum was significantly increased(P<0.01).The thymic naive T cell significant molecule,CD45RA was decreased(P<0.05).T cell activation-related molecules,CD28 and CD25 were both decreased(P<0.05),and PD-1 was significatnly inereased(P<0.01).The memory T cell significant molecule,CD196 was inereased,but was not significantly compared to the control mice.CONCLUSION: The D-galactose subacute aging mice model was successfully established.The naive and active T cell were decreased and the memory T cell was increased in the thymic of the aging.