| 黄连素对巨噬细胞清道夫受体表达的影响 |
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| 引用本文： | 黄周青,蔡雪黎,卢中秋,黄伟剑.黄连素对巨噬细胞清道夫受体表达的影响[J].温州医学院学报,2012,42(2):107-109. |
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| 作者姓名： | 黄周青 蔡雪黎 卢中秋 黄伟剑 |
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| 作者单位： | 1. 温州医学院附属第一医院心血管内科,浙江温州,325000
2. 温州医学院附属第一医院急诊科,浙江温州,325000 |
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| 基金项目： | 浙江省中医药科技局科研基金资助项目,浙江省医学创新学科建设计划项目 |
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| 摘 要： | 目的:探讨黄连素(berberine)对巨噬细胞清道夫受体CD36、LOX-1、CD68等表达的影响.方法:以佛波醇酯(PMA)诱导分化的单核源巨噬细胞为细胞模型,黄连素(50 μmol/L)预处理1h后,加入氧化低密度脂蛋白(oxLDL)刺激,采用流式细胞仪检测巨噬细胞表面清道夫受体的表达.结果:单核细胞分化成巨噬细胞后,CD36表达明显升高,而黄连素预处理显著抑制CD36的表达.oxLDL刺激巨噬细胞24 h后,巨噬细胞表面清道夫受体CD36、LOX-1和CD68表达明显升高(P＜0.01),黄连素预处理后巨噬细胞表面CD36、LOX-1的表达受到抑制,但CD68的表达未受影响(P＞o.05).结论:黄连素抑制巨噬细胞表面清道夫受体CD36、LOX-1的表达,一定程度上可减少巨噬细胞通过清道夫受体途径对脂质oxLDL的吞噬.
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| 关 键 词： | 黄连素 清道夫受体 巨噬细胞 |
Effect of berberine on the expression of scavenger receptors in macrophages |
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| HUANG Zhouqing
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CAI Xueli
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LU Zhongqiu
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HUANG Weijian.Effect of berberine on the expression of scavenger receptors in macrophages[J].Journal of Wenzhou Medical College,2012,42(2):107-109. |
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| Authors: | HUANG Zhouqing CAI Xueli LU Zhongqiu HUANG Weijian |
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| Institution: | .Department of Cardiology,the First Affiliated Hospital of Wenzhou Medical College,Wenzhou,325000 |
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| Abstract: | Objective: To investigate the effect of berberine on the expression of scavenger receptors(CD36,LOX-1,CD68) in macrophages.Methods: PMA-induced macrophages stimulated by oxLDL was used as model in this study to investigate the expression of scavenger receptors,including CD36,CD68 and LOX-1,which was tested using Flow cytometry.Results: Berberine greatly inhibited CD36 expression during monocytes differentiation to macrophages and similar result was obtained in oxLDL-stimulated macrophages.Moreover,in oxLDL-stimu-lated macrophages,LOX-1 expression,but not CD68,was also decreased after berberine treat-ment than those in oxLDL only group.Conclusion: Berberine suppresses the expression of CD36 and LOX-1,but not CD68 in macrophages. |
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| Keywords: | berberine scavenger receptors macrophages |
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