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| HPLC法测定草珊瑚中富马酸含量的方法学研究 | |
| 引用本文： | 谭小鸿,张援虎,张荣祥. HPLC法测定草珊瑚中富马酸含量的方法学研究[J]. 亚太传统医药, 2009, 5(8): 43-46 |
| 作者姓名： | 谭小鸿 张援虎 张荣祥 |
| 作者单位： | 1. 贵州大学,生命科学学院,贵州,贵阳,550025;中国科学院天然产物化学重点实验室,贵州,贵阳,550002 2. 中国科学院天然产物化学重点实验室,贵州,贵阳,550002 3. 贵州大学,生命科学学院,贵州,贵阳,550025 |
| 摘 要： | 目的:建立高效液相色谱测定草珊瑚中富马酸含量的方法。方法:采用高效液相色谱法,固定相为Dia-monsil C18柱(5μm,250×4.6mm),以乙腈-水-磷酸(2:98:0.05)作为流动相,检测波长为208nm。结果:富马酸在54.35~434.8μg范围内线性关系良好,r2=0.9999,平均回收率为99.95%,RSD=1.33%。结论:该方法操作简单、准确,重复性好,可适用于草珊瑚药材的质量控制。 |
| 关 键 词： | 高效液相色谱 草珊瑚 富马酸 含量测定 |
Rearch on the Determination of Fumaric Acid in Sarcandra Glabra (Thunb.) Nakai by HPLC |
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| Tan Xiaohong,Zhang Yuanhu,Zhang Rongxiang. Rearch on the Determination of Fumaric Acid in Sarcandra Glabra (Thunb.) Nakai by HPLC[J]. Asia-Pacific Traditional Medicine, 2009, 5(8): 43-46 | |
| Authors: | Tan Xiaohong Zhang Yuanhu Zhang Rongxiang |
| Affiliation: | (School of Life Science of Guizhou University, Guiyang 550025,China; The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, China) |
| Abstract: | Objective.. To establish a HPLC method for the quantitative determination of fumaric acid in Sarcandra glabra （Thunb.） Nakai. Methods：The determination was carried out with Diamonsil C18 column （ 5μm, 250× 4. 6mm）, using acetoni- trile-water-H3 PO4 （2 ： 98 ：0. 05, V/V） as mobile phase with isocratic elution, at a flow rate of lmL/min and detected at the wave- length 208nm. Results： Fumaric acid had a good linear relationship between 54. 35~434. 8μg （r2 = 0. 9999）,and the average re- covery rate of fumaric acid is 99.95%, RSD is 1.33%. Conclusion.. The method is very simple and accurate, and has good reap- pearance. It can be used for the quality control of Sarcandra glabra （Thunb.） Nakai. |
| Keywords: | HPLC Sarcandra glabra （Thunb.） Nakai Fumaric acid Quantitative determination |
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