食管癌及癌旁组织中端粒酶活性检测及其意义

目的探讨食管癌及癌旁组织中端粒酶活性检测的意义.方法采用PCR技术为基础的TRAP法检测了39例食管癌及癌旁组织端粒酶活性.结果39例食管癌组织中34例端粒酶活性阳性,阳性率为87.2%,癌旁组织中3例阳性,阳性率为7.7%,9例食管良性组织中1例阳性,阳性率为11.1%.食管癌与相应癌旁组织和良性食管组织端粒酶阳性检测率相比,差异有显著性意义(p<0.01).伴有淋巴结转移的25例食管癌端粒酶阳性检测率为96.0%,显著高于未伴有淋巴结转移的阳性率(71.4%),两者差异有显著性意义(p<0.05).结论端粒酶激活与食管癌发生发展有关,对端粒酶活性进行检测对食管癌的诊断和判断预后有重要价值.

Detection of Telomerase Activity in Esophageal Cancer and Tumor-adjacent Tissues

Objective To detect the telomerase activity in esophageal cancer and their tumor-adjacent tissues. Methods Telomerase activity were examined in 39 cases of esophageal cancer and their tumor-adjacent tissues by using PCR-based telomeric repeat amplification protocol assay-TRAP assay. Results 34 of the 39 esophageal cancer (87.2%),3 of 39 tumor-adjacent tissues (7.7%), and 1 of 9 normal esophageal tissues (11.1%) revealed positive for telomerase activity.There are very evident difference of telomerase positive rates among esophageal cancer and their correspond tumor-adjacent tissues and normal esophageal tissues (p<0.01). The positive rates of telomerase activity in 25 esophageal cancer with lymph node metastasis and 14 esophageal cancer without lymph node metastasis were 96.0% and 71.4% respectively (p<0.05). Conclusions The expression of telomerase activity may play an important role in esophageal carcinogenesis and it suggests that examining telomerase activity may be a useful marker in diagnosis and prognosis of esophageal cancer.