| TRF2 siRNA抑制多药耐药胃癌细胞Rapl表达 |
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| 引用本文： | 宁寒冰,王艳红,张连峰,李继昌.TRF2 siRNA抑制多药耐药胃癌细胞Rapl表达[J].中原医刊,2011(17):23-25. |
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| 作者姓名： | 宁寒冰 王艳红 张连峰 李继昌 |
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| 作者单位： | 大学第一附属医院消化科,郑州450052 |
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| 摘 要： | 目的应用RNA干扰技术抑制多药耐药衍生胃癌细胞系TRF2表达，观察其对Rapl表达的影响。方法根据pSilencer3．1-H1载体要求构建TRF2siRNA真核表达载体，分别转染SGC7901／ADR和SGC7901／VCR细胞，免疫荧光技术和Westernblot方法检测Rapl表达。结果成功构建TRF2 siRNA真核表达载体能显著抑制耐药细胞TRF2表达，siRNA转染细胞中Rapl表达明显降低。结论TRF2调控Rapl表达。
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| 关 键 词： | TRF2 Rapl 胃癌 多药耐药 |
Down - regulation of Rap1 expression by TRF2 siRNA in multidrug - resistant gastric cancer cells |
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| NING Han-bing,WANG Yan-hong,ZHANG Lian-feng,LI Ji-chang.Down - regulation of Rap1 expression by TRF2 siRNA in multidrug - resistant gastric cancer cells[J].Central Plains Medical Journal,2011(17):23-25. |
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| Authors: | NING Han-bing WANG Yan-hong ZHANG Lian-feng LI Ji-chang |
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| Institution: | (Department of DigestiveDiseases, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China) |
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| Abstract: | Objective To evaluate the effect of TRF2 siRNA on Rapl expression in muhidrug - resistant gastric cancer cells. Methods TRF2 siRNA eukaryotic expression vector based on pSilencer 3.1 -H1 vector was constructed and transfected into SGC7901/ADR and SGC7901/VCR cells respectively ; the expression of Rapl were detected by immunofluorescence and Western blot. Results The expression of both TRF2 and Rapl was significantly inhibited by TRF2 siRNA in muhidrug - resistant gastric cancer cells. Conclusions TRF2 regulate the expression of Rapl. |
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| Keywords: | TRF2 Rap1 Gastric cancer Multidrug resistance |
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