血清血管内内生长因子在宫颈鳞癌化疗前后的变化

目的探讨血管内皮生长因子（VEGF）能否作为评判宫颈癌化疗近期疗效的预测指标，为制定合理的治疗方案提供参考。方法采用酶联免疫吸附法（ELISA）监测60例宫颈鳞癌患者及20例正常健康妇女血清中的VEGF水平（P〈0．01）。结果宫颈癌I期血清VEGF值低于Ⅱ期者（P〈0．05）；宫颈癌患者术后血清VEGF表达较术前明显上升（P〈0．01），术后化疗后血清VEGF水平较术后明显下降（P〈0．01）；宫颈癌I期患者术后化疗后血清VEGF水平与术前比较差异无统计学意义（P〉0．05），宫颈癌Ⅱa期、IIb期患者术后化疗后血清VEGF水平均低于术前（P〈0．05）；宫颈癌1Ib期患者新辅助化疗（NACT）后血清VEGF浓度较NACT前降低（P〈0．05）。结论VEGF可作为预测宫颈癌化疗近期疗效和判断术后是否需要辅助化疗及化疗疗程的参考指标。

Changes of serum VEGF in cervical squamous cell carcinoma before and after chemotherapy

Objective To explore the feasibility of applying the vascular endothelial growth factor（VEGF） to predicting and evaluating cervical cancer chemotherapy by investigating the changes of VEGF before and after chemotherapy, so as to guide the rational treatment. Methods Using enzyme - linked immunosorbent assay （ELISA）to detect and compare serum VEGF levels in 60 cases of cervical squamous cell carcinoma and 20 healthy women. Results Levels of serum VEGF in cervical carcinoma were significantly higher than those of normal healthy women （ P 〈 0.01 ） , and the serum VEGF values of cervical cancer in stage I were lower than those in stage II （P 〈 0.05）. Expressions of serum VEGF in cervical cancer patients significantly increased after surgery compared with that before surgery （P 〈 0. 01 ）, and decreased significantly after postoperative chemotherapy compared with that after surgery （P 〈 0.01 ）, but the serum VEGF levels in stage I cervical cancer patients after postoperative chemotherapy showed no significant difference compared with that before surgery （P 〈 0.05 ）, while those in stage II a and II b cervical cancer patients after postoperative chemotherapy were lower than those before surgery （P 〈 0. 05 ）. The expressions of serum VEGF in stage IIb cervical cancer patients after neoadju- rant chemotherapy（NACT） were lower than those before NACT （P 〈 0. 05 ）. Conclusions VEGF can be used as an indicator for forecasting chemotherapy effects in cervical cancer and for determining whether to reinforce chemotherapy and postoperative chemotherapy regimen.