荷胃癌小鼠自杀基因治疗后宿主免疫功能观察

目的 研究自杀基因疗法是否能诱导肿瘤宿主产免疫应条。方法 将小鼠胃癌细胞株（ＭＦＣ）接种于小鼠背部制作肿瘤模型，以单线疱疹病毒苷激酶基因（ＨＳＶ－ｔｋ）和大肠杆菌胞嘧啶脱氧基酶基因（ＣＤ）注射于瘤体内，并于瘤体周围注射ｍＩＬ－２和ｍＧＭ－ＣＳＦ ＤＮＡ及向腹腔内注射前药９－丙氧乌苷（ＧＶＣ）和５－Ｆｃ。治疗第５周取肿瘤消退小鼠的脾细胞测自然杀伤细胞（ＮＫ）和ＬＡＫ活性；第１２周取ＰＢＬ和脾细胞测Ｎ

A Study on the Immune Response of Mice with Gastric Cancer Induced by Gene Therapy

Objective To study whether the suicide gene therapy can induce the immune response of the tumor bearer. Methods The tumor model was made by inoculating MFC on the back of the mice. HSV-tk gene and CD gene were injected into the tumor; mIL-2; mGM-CSF DNA were injected around the tumor; prodrugs(GVC and 5-Fc) were injected into the abdominal cavity. In the fifth week after gene treatment, the NK and LAK activities of the spleen cells from the tumor bearer were determined. In the twelfth week, the NK and CTL activities of PBL or spleen cells were tested. Results In the fifth week after gene therapy was administered, the NK and LAK activities of the spleen cells from the gene therapy experimental group were obviously higher than tumor bearer controls(P<0.01). In the twelfth week of treatment, the NK and CTL activities of PBL from experimental mice are enhanced markedly to compare with normal controls(NK, P=0.05; CTL, P<0.01). There is no difference in the NK activity of the spleen cells between experimental group and control group(P>0.05). Conclusion The suicide gene therapy can enhance the antitumor immunity ability for a rather long time.