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| 高效液相色谱法测定活血清脑丸中阿魏酸的含量 | |
| 引用本文： | 郝影霞,王逢春,王焱. 高效液相色谱法测定活血清脑丸中阿魏酸的含量[J]. 华北国防医药, 2012, 24(7): 29-31 |
| 作者姓名： | 郝影霞 王逢春 王焱 |
| 作者单位： | 1. 北京军区总医院263临床部药剂科,北京,101149 2. 北京军区总医院医务处,北京,100700 3. 总参谋部管理保障部旃坛寺门诊部内科,北京,100034 |
| 摘 要： | 目的 应用高效液相色谱法测定活血清脑丸中阿魏酸的含量.方法 色谱柱:Agilent Zorbax SB C18柱(4.6 mm×150 mm,5 μm);流动相:甲醇-1%冰醋酸溶液(30∶70);流速为1.0 ml/min;检测波长为321 nm;柱温为30℃;进样量为10 μl.结果 阿魏酸在2.01～40.16 μg/ml范围内与其峰面积线性关系良好(r=0.9999),平均回收率(n=6)为101.09%,相对标准偏差为1.88%.活血清脑丸供试品(批号20090316、20100719、20101207)中阿魏酸含量分别为125.7、165.2、98.5 μg/g,相对标准偏差分别为1.33%、1.92%、1.37%.结论 高效液相色谱法用于活血清脑丸阿魏酸的含量测定操作简便、准确可靠,有助于加强该制剂的质量控制. |
| 关 键 词： | 活血清脑丸 阿魏酸 色谱法,高效液相 |
Determination about Content of Ferulic Acid in Activating Blood Circulated-Clear Brain Pill by High Performance Liquid Chromatography |
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| HAO Ying-xia , WANG Feng-chun , WANG Yan. Determination about Content of Ferulic Acid in Activating Blood Circulated-Clear Brain Pill by High Performance Liquid Chromatography[J]. Medical Journal of Beijing Military Region, 2012, 24(7): 29-31 | |
| Authors: | HAO Ying-xia WANG Feng-chun WANG Yan |
| Affiliation: | 1.Department of Pharmaceutical Preparation Section,the 263rd Clinical Department,Beijing Military Area Command,Beijing 101149,China;2.Medical Department,Beijing Military Area Command,Beijing 100700,China;3.Department of Internal Medicine,Zhantan Out-patient Department,Management-Security Ministry of General Staff Headquarters,Beijing 100034,China) |
| Abstract: | Objective To use high performance liquid chromatography method（HPLC） for determination about content of ferulic acid in activating blood circulated-clear brain pill.Methods The Agilent Zorbax SB C18 pole（4.6 mm×150 mm,5 μm） was used,the mobile phase consisted of methanol-1% acetic acid（30∶70）,the flow rate was 1.0 ml/min,the detection wavelength at 321 nm,the column temperature at 30℃,and the inject volume 10 μl.Results A good linear correlation of ferulic acid was observed within the range of 2.01-40.16 μg/ml（r=0.9999）.The average recovery was 101.09% with RSD 1.88%（n=6）.The contents of feulic acid in activating blood circulated-clear brain pill（batch 20090316,20100719,20101207） were 125.7,165.2 and 98.5 μg/g with RSD 1.33%,1.92% and 1.37%,respectively.Conclusion The method is simple and accurate and can be used for the determination and quality control of ferulic acid in activating blood circulated-clear brain pill. |
| Keywords: | Activating blood circulated-clear brain pill Ferulic acid Chromatography, high performance liquidchromatography |
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