Studies on the protective effect of dietary fish oil on cisplatin induced nephrotoxicity in rats |
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Authors: | Naqshbandi Ashreeb Khan Md Wasim Rizwan Sana Rehman Sayeed Ur Khan Farah |
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Affiliation: | Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India. |
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Abstract: | Cisplatin (CP) is a major antineoplastic drug for the treatment of solid tumors, however, dose dependent nephrotoxicity remains the major concern for its long term use. Several agents/strategies were attempted to prevent CP nephrotoxicity but were not found suitable for clinical practice. Dietary fish oil (FO) enriched in ω-3 fatty acids has been shown to prevent/reduce the progression of certain types of cancers, cardiovascular and renal disorders. The present study was undertaken to see whether FO can prevent CP-induced nephrotoxic and other deleterious effects. Rats were prefed experimental diets for 10days and then received a single dose of CP (6mg/kg body weight) intraperitoneally while still on diet. Serum/urine parameters, enzymes of carbohydrate metabolism, brush border membrane (BBM) and oxidative stress in rat kidney were analyzed. CP nephrotoxicity was recorded by increased serum creatinine and blood urea nitrogen. CP decreased the activities of metabolic enzymes, antioxidant defense system and BBM enzymes. In contrast, FO alone increased enzyme activities of carbohydrate metabolism and brush border membrane (BBM). FO feeding to CP treated rats markedly enhanced resistance to CP-elicited deleterious effects. Dietary FO supplementation ameliorated CP induced specific metabolic alterations and oxidative damage due to its intrinsic biochemical antioxidant properties. |
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Keywords: | ACPase, acid phosphatase ALP, alkaline phosphatase ALT, alanine aminotranferase AST, aspartate aminotransferase BBM, brush border membrane BBMV, brush border membrane vesicles BUN, blood urea nitrogen CP, cisplatin CPF, cisplatin-fish oil FO, fish oil FBPase, fructose-1,6-bisphosphatase G6Pase, glucose-6-phosphatase G6PDH, glucose-6-phosphate dehydrogenase GGTase, γ-glutamyl transferase GSH-Px, glutathione peroxidase HK, hexokinase LAP, leucine aminopeptidase LDH, lactate dehydrogenase MDH, malate dehydrogenase ME, malic enzyme NADP+, nicotinamide adenine dinucleotide phosphate PUFA, polyunsaturated fatty acids ROS, reactive oxygen species Scr, serum creatinine SOD, superoxide dismutase |
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