A monoclonal antibody raised to rat liver cytochrome P-448 (form C) which recognises an epitope common to many other forms of cytochrome P-450 |
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| Authors: | A R Boobis J McQuade D Sesardic R T Robson C Hayward E A Lock C R Elcombe M S Rose D S Davies |
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| Affiliation: | 2. Department of Clinical Pharmacology, Royal Postgraduate Medical School, London W12 0HS, UK;3. Corporate Bioscience Group, Imperial Chemical Industries PLC, Runcorn, Cheshire WA7 4QE, UK;4. Central Toxicology Laboratory, Imperial Chemical Industries PLC, Alderlley, near Macclesfield, Cheshire SK10 4TJ, U.K.;1. Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China;2. Key Laboratory of Tea Biology and Resource Utilization of Ministry of Agriculture, Hangzhou, 310008, China;1. University of Alberta, Canada;2. University of Texas at Austin, USA;1. Department of Medicinal Chemistry, School of Pharmacy, University of Washington, Seattle, Washington;2. Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California |
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| Abstract: | A murine monoclonal antibody has been raised against a partially purified preparation of hepatic cytochrome P-448 (form c) from beta-naphthoflavone-treated rats. The monoclonal origin of the antibody was established by limiting dilution culture and isoelectricfocusing. The antibody has been designated 3/4/2. It reacts with apparently homogeneous cytochrome P-448 from rat liver in solid phase assay. It also cross reacts with a number of other cytochromes P-450, from rat and rabbit. In addition, a positive reaction was obtained with microsomal fractions from a variety of species, including man. None of the species tested was negative. The antibody does not react appreciably with purified haemoproteins other than cytochromes P-450. Antibody 3/4/2 is not inhibitory, either in reconstituted systems or with intact microsomal fraction. However, evidence was obtained that the antibody does cause some perturbation of the tertiary structure of the apoprotein at or near the haem. |
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