| 小鼠胰岛内分泌细胞的构成及其与Ⅱ型糖尿病的关系 |
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| 引用本文： | 张旭东,贾书花,张雷. 小鼠胰岛内分泌细胞的构成及其与Ⅱ型糖尿病的关系[J]. 解剖科学进展, 2009, 15(1) |
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| 作者姓名： | 张旭东 贾书花 张雷 |
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| 作者单位： | [1]长治医学院组织胚胎学教研室,山西长治046000 [2]河北医科大学组织胚胎学教研室,河北石家庄050017 |
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| 摘 要： | 目的观察和分析II型糖尿病动物模型db/db小鼠病程进展中胰岛内分泌细胞的构成变化及与II型糖尿病病因的关系。方法分别选取3、5、8、10和12月龄的尾静脉空腹血糖高于10.0mmol/L且肥胖的db/db自发性糖尿病小鼠,每组5只,作为糖尿病组;选取相应年龄段的尾静脉空腹血糖低于6.0mmol/L体重正常的db/+m表型正常小鼠,每组5只,作为对照组。于相应年龄段取胰尾,用于免疫组织化学观察和比较。结果各月龄糖尿病组胰岛B细胞(胰岛素阳性细胞)阳性率低于对照组(p<0.05),而A细胞(胰高血糖素阳性细胞)和D细胞(生长抑素阳性细胞)阳性率分别高于对照组(p<0.05)。糖尿病组胰岛B细胞阳性率随自发性db/db糖尿病小鼠病程进展呈降低趋势(p<0.05),而A细胞和D细胞阳性率则呈增高趋势(p<0.05);对照组3种细胞阳性率无变化(P>0.05)。结论II型糖尿病动物模型db/db小鼠胰岛内分泌细胞的构成变化与糖尿病病情的轻重有关,胰岛内分泌细胞之间的比例失衡可能与II型糖尿病的发病有关。
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| 关 键 词： | 糖尿病 胰岛 内分泌细胞 免疫组织化学 小鼠 |
The Constitution Changes of Pancreatic Islet Endocrine Cells and their Relation to Type-2 Diabetes in Mice |
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| ZHANG Xu-Dong ,JIA Shu-Hua ,ZHANG Lei. The Constitution Changes of Pancreatic Islet Endocrine Cells and their Relation to Type-2 Diabetes in Mice[J]. Progress of Anatomical Sciences, 2009, 15(1) |
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| Authors: | ZHANG Xu-Dong JIA Shu-Hua ZHANG Lei |
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| Affiliation: | 1.Department of Histology and Embryology;Changzhi Medical College;Shanxi Changzhi 046000;2.Department of Histology and Embryology;Hebei Medical University;Hebei Shijiazhuang 050017 China |
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| Abstract: | To observe and analyse the quantity changes of pancreatic islet endocrine cells in spontaneous db/db diabetic mice and explore their relationship with pathogeny of type 2 diabetes mellitus.Methods 3,5,8,10,12 month-old obese db/db mice with fasting blood glucose(FBG) more than 10.0mmol/L were selected(25mice),the age-matched normal weight db/+m mice with FBG less than 6.0mmol/L as the control group(25mice).The tails of pancreas were excised and observed by immunohistochemical staining.Results The positive r... |
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| Keywords: | Diabetes mellitus pancreatic islet endocrine cells immunohistochemistry mice |
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