负性急性时相蛋白在慢性荨麻疹患者的研究

目的 探讨急性时相反应与慢性荨麻疹的关系。 方法 慢性荨麻疹患者50例，按症状分为轻型（1级）、中型（2级）、重型（3级）3组，同时选取健康体检者28例，用免疫比浊法测定血清前白蛋白（PA）、转铁蛋白（TRF）水平，采用酶联免疫吸附法测定胰岛素样生长因子1（IGF-1）和肿瘤坏死因子α（TNF-α）水平；比较两组之间的差异、各检测项目之间的相关性及与病情分级的关系。 结果 慢性荨麻疹患者PA（229.99 ± 54.16） mg/L、IGF-1（177.23 ± 46.48） μg/L均明显低于健康对照组，两组差异有统计学意义（均P < 0.05）。TNF-α（25.39 ± 11.01） ng/L高于健康对照组（14.13 ± 6.12） ng/L，差异有统计学意义（P < 0.05）。TRF水平（2.48 ± 0.49） g/L与健康对照组（2.48 ± 0.25） g/L比较，差异无统计学意义（P > 0.05）。血清PA与TNF-α呈显著负相关（P < 0.05），并与病情严重程度呈显著负相关（P < 0.01），而TNF-α则与病情严重程度呈显著正相关（P < 0.01）；其余各指标之间无明显相关性（均P > 0.05）。 结论 在慢性荨麻疹患者中，部分急性时相反应负性蛋白水平降低且与病情呈负相关，急性时相反应可能参与慢性荨麻疹的发病。

Negative acute phase proteins in patients with chronic urticaria

Tang Huimin, Yang Wenlin*, Huang Taoyuan, Yin Jiawen, Yang Jian. *Department of Dermatology, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China Corresponding author: Yang Wenlin, Email: yangwenlin@21cn.com 【Abstract】 Objective To investigate the relationship between negative acute phase proteins and chronic urticaria （CU）. Methods Fifty patients with CU were enrolled into this study, and divided into three grades, i.e., mild （grade 1）, moderate （grade 2） and severe （grade 3） according to symptoms. Twenty-eight health checkup examinees served as the control group. Immunoturbidimetry was performed to determine serum levels of prealbumin （PA） and transferrin （TRF）. Enzyme-linked immunosorbent assay was conducted to measure serum levels of insulin-like growth factor-1 （IGF-1） and tumor necrosis factor-α （TNF-α）. Statistical analysis was carried out to assess differences in these indices between these two groups, the relationship among these indices and between these indices and disease severity. Results Compared with the control group, the patients with CU showed reduced serum levels of PA （（229.99 ± 54.16） vs. （272.06 ± 36.42） mg/L, t =3.667, P < 0.05） and IGF-1 （（177.23 ± 46.48） vs. （239.88 ± 45.16） μg/L, t = 5.748, P < 0.05）, but higher serum levels of TNF-α （（25.39 ± 11.01） vs. （14.13 ± 6 .12） ng/L, t = 4.989, P < 0.05）, and similar serum levels of TRF （（2.48 ± 0.49） vs. （2.48 ± 0.25） g/L, P > 0.05）. The serum level of PA showed a significant negative correlation with that of TNF-α （r = － 0.312, P < 0.05）, as well as with disease severity （r =－ 0.635, P < 0.01）, whereas the serum level of TNF-α showed a significant positive correlation with disease severity （r = 0.409, P < 0.01）, and no statistical correlation was found between the remaining indices （all P > 0.05） in the patients with CU. Conclusions Serum levels of some negative acute phase proteins decrease and negatively correlate with disease severity in patients with CU. Acute phase response may be involved in the occurrence of CU.