糖尿病对大鼠牙槽骨缺损修复中骨形态发生蛋白-2表达影响的研究

目的 观察糖尿病(diabetes mellitus,DM)对实验性大鼠牙槽骨缺损修复过程中不同时期骨形态发生蛋白-2(bonemorphogenetic protein-2,BMP-2)表达的影响。方法 将48只雄性SD大鼠随机分为DM组和对照组,每组24只,DM组大鼠经腹腔注射链脲佐菌素造成DM大鼠模型,建模成功后行大鼠牙槽骨骨缺损制备,2组均分别于骨缺损制备后1、2、4、8周各取6只大鼠处死,取术区组织。苏木精-伊红染色(hematoxylin-eosin staining,HE染色)镜下观察缺损区内新生骨样组织形成情况;用免疫组化法检测术后1、2、4、8周缺损区内BMP-2的表达情况,比较各组的平均光密度。结果 HE染色观察显示DM组成骨较对照组减少。术后1周、2周免疫组化观察对照组新骨BMP-2的表达强于DM组,二者之间差异有统计学意义(P<0.05),术后4周、8周对照组BMP-2表达较之前弱,但与DM组二者之间差异无统计学意义(P>0.05)。结论 DM影响了大鼠牙槽骨缺损的修复,可能是DM使BMP-2形成减少,从而抑制了未分化间充质向成骨细胞的转化,从而影响种植体骨结合,降低种植初期稳定性。?

Effects of diabetes on expression of BMP-2 during bone healing of alveolar defect in rats

Objective To investigate the effects of diabetes on the expression of bone morphogenetic protein-2（BMP-2） during the various bone healing periods of alveolar defect.Methods 48 male SD rats were randomly divided into diabetes group（n=24） and control group（n=24）.Diabetic rats were induced by intraperitoneal injection of streptozotocin（STZ）,and alveolar defect was created through the diabetic duration time.6 rats in each group were scarified respectively at the 1th,2th,4th,8th week,then the alveolar bone were processed for histological examination.The expression of BMP-2 in different periods（1,2,4,8 weeks）of alveolar defect healing of rats was investigated through immunohistochemistry method.The optical density（OD） of BMP-2 was analysed and compared between groups.Results Osteopenia in diabetes group were observed.The OD of BMP-2 in the control group was statistically greater than that in the test group 1 and 2 weeks after alveolar defect.After 4 and 8 weeks,the expression of BMP-2 in the control group decreased,and no statistical difference was found in BMP-2 expression between these two groups.Conclusions Diabetes may affect the formation of BMP-2,leading to a reduction in bone healing.Diabetes is able to affect the differentiation of mesenchymal stem cells,leading to less osseointegration.Therefore,primary stability was decreased.