Safety assessment of transgenic canola RF3 with bar and barstar gene on Sprague-Dawley (SD) rats by 90-day feeding test |
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| Affiliation: | 1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China;2. Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, 100083, China;3. Beijing Laboratory for Food Quality and Safety, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China;1. China Agricultural University, Supervision and Testing Center for Genetically Modified Organisms Food Safety, Ministry of Agriculture, Beijing, China;2. Embrapa Swine and Poultry, Concórdia, Santa Catarina, Brazil;3. BASF Corporation, 26 Davis Drive, Research Triangle Park, NC 27709, USA;4. BASF Plant Science LP, 26 Davis Drive, Research Triangle Park, NC 27709, USA;5. BASF China, 25/F, Tower A, Gateway Plaza, 100027 Beijing, China;6. BASF South America, Av. das Nações Unidas, 14.171, 14° andar, 04794-000 Sao Paulo, Brazil;1. Food & Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India;2. Biomedical Informatics Centre(BIC), National Institute of Nutrition(NIN), Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India;3. Division of Biostatistics, National Institute of Nutrition, Indian Council of Medical Research (ICMR), Jamai Osmania P.O., Hyderabad 500007, Telangana, India;4. Centre for Genetic Manipulation of Crop Plants, University of Dehli South Campus, New Delhi 110021, India;5. Food Allergy Research and Resource Program, Dept. of Food Science & Technology, University of Nebraska—Lincoln, Lincoln 68583-0955, NE, USA;1. Dow AgroSciences LLC, Indianapolis, IN 46268, USA;2. Toxicology and Environmental Research and Consulting, The Dow Chemical Company, Midland, MI 48674, USA;1. Department of Environmental Biology, Sapienza University of Rome, Via dei Sardi 70, 00185 Roma, Italy;2. Department of Crop and Forest Sciences, Agrotecnio Centre, Universitat de Lleida, Av Rovira Roure 191, 25198 Lleida, Spain |
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| Abstract: | Canola is one of the most important plant oilseed crops. To avoid the threat of herbicides, the RF3 line with bar gene and barstar gene was developed, which can act as glufosinate resistance resources and restore fertility in hybrid lines. To assess the food safety of transgenic canola RF3, 2.5%, 5% and 10% GM canola RF3 and its non-GM isogenic line Drakkar were formulated into diet to feed Spragure-Dawley (SD) rats for 90 days. The effects on the general growth and toxicological parameters, as well as gut microbiota of rats, were evaluated. Several significant differences on body weight, feed consumption, relative organ weight, hematology and serum biochemistry were observed among rats in the 90-day feeding test. However, these statistical differences were randomly observed among different groups and were not dose-related, which were not considered to be biologically significant. Furthermore, the results of bacterial 16S rRNA sequencing of fecal samples showed that the diets containing GM canola did not disturb the balance of gut microbiota. In conclusion, the canola RF3 is considered as safe and wholesome as the non-GM canola based on this 90-day feeding test and gut microbiota analysis. |
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| Keywords: | Transgenic canola Safety assessment 90-Day feeding test Gut microbiota |
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