Oral mucosal irritation study in hamster to evaluate a therapeutic apparatus using hydrogen peroxide photolysis for periodontitis treatment |
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| Affiliation: | 1. Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan;2. Hatano Research Institute, Food and Drug Safety Center, 729-5 Ochiai, Hadano, Kanagawa 257-8523, Japan;1. Centro de Pesquisas em Biologia Molecular e Funcional (CPBMF), Instituto Nacional de Ciência e Tecnologia em Tuberculose (INCT-TB), Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681, Prédio 92A Tecnopuc, 90619-900, Porto Alegre, Brazil;2. Programa de Pós-Graduação em Medicina e Ciências da Saúde, PUCRS, Porto Alegre, Brazil;3. Programa de Pós-Graduação em Biologia Celular e Molecular, PUCRS, Porto Alegre, Brazil;4. Departamento de Farmacologia, Universidade Federal de Santa Catarina, Florianópolis, Brazil;5. Instituto de Toxicologia e Farmacologia, PUCRS, Porto Alegre, Brazil;1. Department of Dentistry, the Affiliated Yan''an Hospital of Kunming Medical University, Kunming, Yunnan, China;2. Department of Periodontology, the Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan, China;3. Department of Endodontology, the Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan, China;4. Department of Dentistry, Wuxi Mental Health Center, Jiang Su, China;1. Graduate School of Peking Union Medical College, Beijing, People''s Republic of China;2. Reproductive Physiology Laboratory, National Research Institute for Family Planning, Beijing, People''s Republic of China;3. Reproductive Medcine Department, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, People''s Republic of China;1. Department of Oral Pathology and Microbiology, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune 411018, MH, India;2. Dr. D.Y. Patil Institute of Biotechnology and Bioinformatics, Dr. D.Y. Patil Vidyapeeth, Pimpri, Pune 411018, Maharashtra, India;3. Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Saudi Arabia |
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| Abstract: | We conducted an oral mucosal irritation study in hamsters to evaluate a therapeutic apparatus using hydrogen peroxide (H2O2) photolysis for periodontitis treatment (ISO 10993-Part 10, Annex B.3). The cheek pouches in 15 male hamsters were allocated to one of six groups. Group 1 received pure water treatment (control group). Group 2 received laser irradiation at 80 mW. Group 3 received 3% H2O2. Groups 4–6 received laser irradiation of 3% H2O2 at 80, 40, and 20 mW, respectively. The total treatment time was set at 7 min and treatment was repeated three times at approximately 1-h intervals. Macroscopic and microscopic histologic observations of the treated sites were performed immediately after each treatment and/or 24 h after the last treatment. The mean scores in macroscopic and histologic examinations in all six groups were 0. Accordingly, irritation indices calculated by subtracting the mean score in each experimental group from that in the control group (Group 1) were 0. Our results suggest that treatment with the apparatus has no mucosal irritation potential in hamster cheek pouches under test conditions simulating clinical conditions. |
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| Keywords: | Hydrogen peroxide Photolysis Hydroxyl radicals Periodontal therapy Oral mucosa irritation Hamster GLP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" good laboratory practice ISO" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" International Organization for Standardization |
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