噬菌体随机肽库中与受损内皮细胞特异性结合短肽的筛选

目的　筛选与受损内皮细胞特异性结合的短肽。方法　以氧化型低密度脂蛋白 (oxidizedlowdensitylipoprotein ,ox -LDL)损伤牛胸主动脉内皮细胞 ,用丝状噬菌体 (FUSE5 )表面展示的随机 6肽和 15肽文库进行混合筛选 ,通过DNA序列测定确定其插入的氨基酸序列。结果　经过 3轮筛选 ,得到一些特异性结合的噬菌体克隆 ,随机挑选 10个克隆 ,进行DNA序列分析 ,其中 8个克隆含 6肽序列 ,2个克隆含 15肽序列。结论　用噬菌体技术能筛到与受损内皮细胞亲和力高、结合力强的特异性短肽

Screening the Peptides Specifically Binding the Injured Endothelial Cell with Two Phage-Displayed Random Peptide Libraries

Objective:To screen the peptides specifically binding the injured endothelial cell(EC) by means of both 6-mer and 15-mer peptides libraries displayed on the surface of filamentous bacteriophage(FUSE5).Methods:Cultured calf aortic endothelial cells were injured by incubating with oxidized low density lipoprotein(ox-LDL) for 4 hours.The binding phage to injured EC was obtained by three rounds of screening and the inserted amino acid sequences of displayed peptides was confirmed by DNA sequences.Results:We primarily isolated ten phage clones from two phage display libraries.Analysis of peptide shows that eight clones include 6-mer sequence and two clones contain 15-mer sequence.Conclusion:We obtained the peptides specifically binding with high avidity to injured EC by the phage-displayed approach.