乌拉坦对大鼠胰岛素水平的影响(英文)

目的　观察麻醉剂量的乌拉坦对空腹大鼠、葡萄糖负荷大鼠和肾上腺素诱发高血糖大鼠血浆胰岛素水平的影响。方法　采用放射免疫测定法测定血浆胰岛素活性。结果　麻醉剂量的乌拉坦 (1 .5g·kg-1 ,sc ,ip各半 )给药后 2 0min显著升高空腹大鼠及葡萄糖负荷大鼠血浆胰岛素水平 ,给药后 50min血浆胰岛素水平分别从 (1 1± 4)及 (1 1± 4)mU·L-1 升至 (2 5±1 1 )及 (47± 6)mU·L-1 ;相同剂量的乌拉坦给药后 1 4 0min使肾上腺素诱发的高血糖大鼠血浆胰岛素水平从 (1 0± 3)升至 (51± 1 8)mU·L-1 。与血浆胰岛素水平的变化相比 ,乌拉坦给药后 50min显著升高空腹大鼠和葡萄糖负荷大鼠的血糖水平 ,但对肾上腺素诱发的高血糖大鼠的血糖水平无明显影响。结论　乌拉坦显著升高空腹大鼠、葡萄糖负荷大鼠及肾上腺素诱发的高血糖大鼠血浆胰岛素水平。乌拉坦促进胰岛素分泌的机制包括高血糖依赖性促分泌和非血糖依赖性促分泌两种

Effect of urethane on insulin level in rats

AIM In view of the contradictory reports in literatures, to further investigate effects of urethane on the plasma insulin levels in the fasting, glucose-loaded and the epinephrine-induced hyperglycemic rats. METHODSPlasma insulin was measured with the radio-immunoassay method. RESULTS Urethane at anesthetic dose (1.5 g*kg-1) significantly increased the plasma insulin levels at 20 min. The plasma insulin levels increased from (11±4) to (25±10) mU*L-1 (P<0.05) in fasting rats and from (11±4) to (47±6) mU*L-1 (P<0.01) in glucose-loaded rats at 50 min after administration of urethane. In hyperglycemic rats induced by epinephrine, urethane significantly increased the plasma insulin from (10±3) to (51±18) mU*L-1 at 140 min after administration of epinephrine and urethane together. In contrast with the changes in plasma insulin levels, urethane significantly increased the blood glucose levels in fasting or glucose-loaded rats at 50 min after administration of urethane, and it did not change the hyperglycemia induced by epinephrine. CONCLUSION Urethane increases the plasma insulin levels in the fasting, glucose-loaded and the epinephrine-induced hyperglycemic rats. Insulin secretion promoted by urethane may via both hyperglycemia-dependent and hyperglycemia-independent mechanisms.