耐甲氧西林金黄色葡萄球菌SCC mec中ccr复合体基因型分析

目的 了解MRSA临床株葡萄球菌盒染色体,mec(SCCmec)中盒染色体重组酶(ccr)复合体型别的特点.方法 收集MRSA临床株78株,应用多重引物PCR扩增ccr复合体各型中的特异性序列,确定ccr型别.结果 78株MRSA中;1株ccr1(1.28%)、3株ccr2(3.85%)、11株ccr3(14.10%)、3株ccr5(3.85%).52株同时携带两种及以上ccr基因,其中50株携带ccr3、ccr5(64.10%)、1株携带ccr2、ccr5(1.28%)、1株携带ccr2、ccr3、ccr5(1.28%),另有8株未检测到ccr基因.结论 本研究中MRSA的ccr基因型以ccr3、ccr5复合型为主,并可能存在一些新型ccr复合体.

Study on ccr gene complex of SCCmec element in methicillin resistant Staphylococcus aureus

Objective To study the genotypic characteristics of ccr gene complex of SCCmec element in clinical isolates of methicillin resistant Staphylococcus aureus (MRSA). Method 78 clinical MRSA. isolates were collected for oar genotyping by multiplex PCR. Results Among 78 MRSA isolate,isolates carried both type3 ccr and type5 ccr(n= 50, 64.10%)were widely distributed. Six minor types were also detected, namely type 1 ccr (n=1,1.28%), type2 ccr (n=3,3.85%), type 3 ccr (n=11, 14.10%), type 5 ccr (n=3,3.85%), type 2 ccr and 5 ccr(n=1, 1.28%), type 2 ccr, type 3 ccr and type 5 ccr (n=1,1.28%). None of ccr gene complex could be detected in 8 isolates. Conclusions Type 3 car and 5 ccr in isolates are the predominant genotypes of ccr gene complex circulating in this study. In addition, some new types of ccr gene complex may exist.