盆腔炎动物模型的建立

目的建立临床盆腔炎的动物模型。方法以健康成年新西兰兔为研究对象将大肠埃希菌接种到输卵管系膜及注射到输卵管,7d后剖腹探查,通过肉眼、光镜及电镜观察炎症形成情况。结果盆腔腹膜和盆腔器官明显充血、水肿、粘连。光镜见明显的炎细胞浸润,电镜下细胞核核膜不清,染色体线粒体肿胀。结论3×108cfu/kg大肠埃希菌注射至输卵管可建立典型的盆腔炎动物模型,其重复性强,可用于实验研究。

Establishment of an Animal Model of Pelvic Inflammation

Objective To establish an animal model with a close similarity of the clinical pelvic inflammation.Mathods Healthy adult New Zealand rabbits were used in this experiment.Escherichia coli was inoculated on the surface of the mesosalpinx and injected into fallopian tubes.7 days later,the degree of pelvic inflammation was measured by gross observation,light microscopy,and electron microscopy.Results Pelvic marked hyperaemia,oedema,purulent discharge and adhesion were observed in peritoneum and pelvic organs.Histologic evaluation revealed significant peritoneal inflammation.Conclusion A reproducible animal model of pelvic inflammation could be established and used in experiment reseach.