临沂地区女性宫颈鳞癌和癌前病变组织中高危型HPV感染状况分析

目的 分析临沂地区宫颈鳞癌和癌前病变组织中高危型人乳头瘤病毒(HR-HPV)的感染状况和分布特点,以期为临沂地区宫颈癌防治和疫苗接种提供一定的理论依据.方法 选取2017年2月—2018年9月临沂市人民医院病理科诊断的宫颈鳞癌和癌前病变活检或手术切除标本191例,应用聚合酶链式反应(PCR)结合Taqman技术对其进行...

Analysis on high-risk human papillomavirus infection in cervical squamous cell carcinoma tissue and precancerous lesion tissue in Linyi region

Objective To analyze the infection status and distribution characteristics of high-risk human papillomavirus(HR-HPV)in cervical squamous cell carcinoma tissue and precancerous lesion tissue in Linyi region,provide a certain theoretical basis for prevention and treatemnt of cervical cancer and vaccination in Linyi region.Methods From February 2017 to September 2018,191 specimens of cervical squamous cell carcinoma tissue and precancerous lesion tissue after biopsy or surgical resection were selected,and polymerase chain reaction(PCR)combined with Taqman technique were used for 15 kinds of high-risk HPV genotyping.Results Among 191 cases,164 cases were found with positive HR-HPV,and the total infection rate was 85.86%,which increased with aggravation of cervical lesions.The infection rate in cervical squamous cell carcinoma group was the highest(53/56,94.64%),and the infection rates in low-grade squamous intraepithelial lesion(LSIL)group and high-grade squamous intraepithelial lesion(HSIL)group were 67.92%(36/53)and 91.46%(75/82),respectively.There was statistically significant difference in infection rate of HR-HPV between LSIL group and HSIL group(χ²=12.203,P=0.000),and also between LSIL group and cervical squamous cell carcinoma group(χ²=12.974,P=0.000).The top five HPV types with infection frequency from high to low were HPV16(107/208,51.44%),HPV58(21/208,10.10%),HPV52(14/208,6.73%),HPV18(12/208,5.77%),and HPV31(11/208,5.29%).Among the cases under 30 years old,the proportion of LSIL was the highest(59.09%).The proportions of HSIL in 30-39-year-old group,40-49-year-old group,and 50-59-year-old group were the highest,which were 48.15%,40.00%,and 46.67%,respectively.In≥60-year-old group,the proportion of cervical squamous cell carcinoma was the highest(66.67%).Among 164 cases with positive HR-HPV,single type HPV infection was the main type,and the rate of single type HPV infection was the highest in HSIL group(64/75,85.33%).Conclusion The most common HR-HPV infection subtypes in cervical squamous cell carcinoma tissue and precancerous lesion tissue in Linyi region were HPV16,58,52,18,and 31,respectively.HR-HPV infection is closely correlated with occurrence and development of cervical squamous cell carcinoma and precancerous lesion,especially HPV16,58,health managemnt of the population should be enahnced.