小分子醇类对磷脂水合和脂质体形成的影响研究EI北大核心CSCD

脂质体由于组分可控,是自然生物膜的良好替代模型。磷脂分子发生水合作用形成规则的膜结构是脂质体形成的先决条件。本文以乙醇分子为模型,考察了小分子醇类对磷脂水合(包括水合过程和水合结果)与脂质体形成的影响,以及醇分子与氯化钠共存时的影响。实验发现,磷脂膜水合过程中乙醇与氯化钠的作用恰好相反。氯化钠会抑制脂质体形成,而乙醇则在一定浓度范围内促进脂质体形成。对比不同乙醇及氯化钠含量时膜上荧光强度和膜连续度的变化,发现当二者同时出现在磷脂膜所处液体环境时,两者对磷脂水合起叠加作用。本研究结果可为人工生物膜的制备提供参考信息。

Study on the effect of small alcohol on lipid hydration and liposome formation

Liposomes with precisely controlled composition are usually used as membrane model systems to investigate the fundamental interactions of membrane components under well-defined conditions.Hydration method is the most common method for liposome formation which is found to be influenced by composition of the medium.In this paper,the effects of small alcohol(ethanol)on the hydration of lipid molecules and the formation of liposomes were investigated,as well as its coexistence with sodium chloride.It was found that ethanol showed the opposite effect to that of sodium chloride on the hydration of lipid molecules and the formation of liposomes.The presence of ethanol promoted the formation of liposomes within a certain range of ethanol content,but that of sodium chloride suppressed the liposome formation.By investigating the fluorescence intensity and continuity of the swelled membranes as a function of contents of ethanol and sodium chloride,it was found that sodium chloride and ethanol showed the additive effect on the hydration of lipid molecules when they coexisted in the medium.The results may provide some reference for the efficient preparation of liposomes.