电场刺激联合聚乙二醇治疗大鼠脊髓损伤的实验研究EI北大核心CSCD

外加直流电场刺激(EFS)可抑制脊髓损伤(SCI)后损伤组织局部Ca2+内流,有效抑制脊髓继发性损伤的发生。但电场刺激结束后,损伤局部Ca2+会重新开始内流并激发后续病理生理学连锁反应,影响了EFS治疗SCI的效果。聚乙二醇(PEG)是一种亲水性的高分子材料,具有促进细胞膜融合及受损细胞膜修复的作用。本文旨在研究EFS联合PEG治疗Sprague-Dawley(SD)大鼠SCI的效果。脊髓损伤后的SD大鼠被随机分为对照组(无治疗,n=10)、电场组(EFS治疗30 min,n=10)、PEG组(浸润50%PEG的明胶海绵覆盖损伤处5 min,n=10)和联合组(电场刺激和PEG联合治疗,n=10)。术后不同时间进行运动诱发电位(MEP)测量、运动行为学评分以及脊髓切片染色分析。研究结果表明,术后8周联合组大鼠MEP潜伏期差和波幅差以及脊髓空洞面积比均显著低于对照组、电场组和PEG组,而运动功能评分和脊髓神经组织残余面积比均显著高于对照组、电场组和PEG组。以上结果提示,联合治疗方法能减轻大鼠脊髓损伤后的病理损伤程度,促进大鼠电生理及运动功能的恢复,其疗效优于EFS和PEG单独使用时的效果。

Experimental study of electric field stimulation combined with polyethylene glycol in the treatment of spinal cord injury in rats

Electric field stimulation(EFS)can effectively inhibit local Ca2+influx and secondary injury after spinal cord injury(SCI).However,after the EFS,the Ca2+in the injured spinal cord restarts and subsequent biochemical reactions are stimulated,which affect the long-term effect of EFS.Polyethylene glycol(PEG)is a hydrophilic polymer material that can promote cell membrane fusion and repair damaged cell membranes.This article aims to study the combined effects of EFS and PEG on the treatment of SCI.Sprague-Dawley(SD)rats were subjected to SCI and then divided into control group(no treatment,n=10),EFS group(EFS for 30 min,n=10),PEG group(covered with 50%PEG gelatin sponge for 5 min,n=10)and combination group(combined treatment of EFS and PEG,n=10).The measurement of motor evoked potential(MEP),the motor behavior score and spinal cord section fast blue staining were performed at different times after SCI.Eight weeks after the operation,the results showed that the latency difference of MEP,the amplitude difference of MEP and the ratio of cavity area of spinal cords in the combination group were significantly lower than those of the control group,EFS group and PEG group.The motor function score and the ratio of residual nerve tissue area in the spinal cords of the combination group were significantly higher than those in the control group,EFS group and PEG group.The results suggest that the combined treatment can reduce the pathological damage and promote the recovery of motor function in rats after SCI,and the therapeutic effects are significantly better than those of EFS and PEG alone.