| 胆囊收缩素对人胆管癌细胞粘附、运动及侵袭能力的影响 |
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| 引用本文： | 张丰深,郑秀海,马宽生,王曙光. 胆囊收缩素对人胆管癌细胞粘附、运动及侵袭能力的影响[J]. 中华消化外科杂志, 2005, 4(4): 258-260 |
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| 作者姓名： | 张丰深 郑秀海 马宽生 王曙光 |
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| 作者单位： | 1. 400020,重庆市江北区解放军324医院普通外科
2. 400038,第三军医大学西南医院全军肝胆外科研究所 |
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| 基金项目： | 重庆市科委应用基础研究基金资助项目(No.20038043) |
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| 摘 要： | 目的研究胆囊收缩素对胆管癌细胞的粘附、运动以及侵袭行为的影响。方法体外培养人胆管癌细胞系QBC939,应用粘附试验、Transwell趋化运动和侵袭模型,研究CCK8S及其不同浓度下对胆管癌细胞的粘附、运动以及侵袭能力的影响。结果CCK8S能够以浓度依赖方式降低QBC939细胞的粘附,而促进其运动和侵袭能力,其受体拮抗剂L18和L60能够明显减弱CCK8S抑制QBC939细胞粘附的作用以及促进瘤细胞运动和侵袭的能力,细胞运动和侵袭实验显示L60具有较强的拮抗CCK8S作用的能力。结论CCK8S具有降低QBC939细胞的粘附、促进QBC939运动和侵袭的能力,CCK2受体在影响QBC939细胞的运动、侵袭能力方面可能具有较强的作用。
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| 关 键 词： | 胆管癌 胆囊收缩素 肿瘤转移 |
| 文章编号： | 1671-4555(2005)04-0258-03 |
| 修稿时间： | 2005-01-19 |
Effect of cholecystokinin on cell adhesion, migration and invasion of human bile duct carcinoma |
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| Zhang Fengshen,Zheng Xiuhai,Ma kuansheng,Wang Shuguang. Effect of cholecystokinin on cell adhesion, migration and invasion of human bile duct carcinoma[J]. Chinese Journal of Digestive Surgery, 2005, 4(4): 258-260 |
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| Authors: | Zhang Fengshen Zheng Xiuhai Ma kuansheng Wang Shuguang |
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| Abstract: | Objective To investigate the effects of cholecystokinin on cell adhesion, migration and invasion of human bile duct carcinoma cell line QBC_(939). Methods Varying concentrations of CCK_(8S) and its receptor antagonists L18 and L60 were used to investigate their effects on adhesion, migration and invasion of QBC_(939). Results CCK_(8S) reduced the ability of adhesion and increased the abilities of migration and invasion of QBC_(939) in dose-dependent manner. L18 and L60 reversed the effect of CCK_(8S), and L60 had stronger effect than L18 on migration and invasion. Conclusions CCK_(8S) could reduce the ability of adhesion and increase the abilities of migration and invasion of QBC_(939) in dose-dependent manner. CCK-2 receptor plays more significant role than CCK-1 in modulating migration and invasion of QBC_(939). |
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| Keywords: | bile duct carcinoma cholecystokinin metastasis |
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